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Team:Carnegie Mellon/Notebook/5 June 2012
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== Inoculation of cells == | == Inoculation of cells == | ||
* Took 1 colony of cells from DNP and Charlie respectively and inoculated in 3 ml of LB+Amp media | * Took 1 colony of cells from DNP and Charlie respectively and inoculated in 3 ml of LB+Amp media | ||
| - | * Left in 37 degree incubator for incubation till next morning | + | * Left in 37 degree incubator for incubation till next morning |
| + | |||
| + | === Comments: === | ||
| + | None | ||
Revision as of 23:04, 26 June 2012
June 05, 2012: 09:30
Contents |
Observations of Plates
- Done after spreading transformed cells on LB+Amp plates overnight
| Name | #Colonies: Plate 1 | #Colonies: Plate 2 |
|---|---|---|
| Charlie | 1 | 0 |
| dNP138 | 7 | 3 |
| - Control | 0 | 0 |
| + Control | TMTC (>200) | TMTC (>200) |
- Note: Charlie only had 1 colony, photo was taken after picking up colony
- Taken using Red Gel Documentation System from Westburg
Comments:
Successful transformation, negative control has none, positive control has TMTC. However, plasmid concentrations/purity are significantly lower than expected.
June 05, 2012: 16:00
Inoculation of cells
- Took 1 colony of cells from DNP and Charlie respectively and inoculated in 3 ml of LB+Amp media
- Left in 37 degree incubator for incubation till next morning
Comments:
None
