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Team:Carnegie Mellon/Notebook/5 June 2012
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| - | '''June 05, 2012:''' | + | <font size=5> |
| - | + | '''June 05, 2012: 09:30 ''' | |
| - | + | </font> | |
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| + | == Observations of Plates == | ||
| + | * Done after spreading transformed cells on LB+Amp plates overnight | ||
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| - | [[File:Carnegie_05_June_2012_im1.jpg | + | [[File:Carnegie_05_June_2012_im1.jpg | LB+Amp Control Groups | border | 500px]] |
| + | <br/><br/> | ||
| + | [[File:Carnegie_05_June_2012_im2.jpg | LB+Amp Charlie Group | border | 500px]] | ||
| + | |||
| + | * Note: Charlie only had 1 colony, photo was taken after picking up colony | ||
| + | * Taken using Red Gel Documentation System from Westburg | ||
| + | |||
| + | |||
| + | === Comments: === | ||
| + | Successful transformation, negative control has none, positive control has TMTC. However, plasmid concentrations/purity are significantly lower than expected. | ||
| + | |||
| + | |||
| + | <font size=5> | ||
| + | '''June 05, 2012: 16:00 ''' | ||
| + | </font> | ||
| + | |||
| + | == Inoculation of cells == | ||
| + | * Took 1 colony of cells from DNP and Charlie respectively and inoculated in 3 ml of LB+Amp media | ||
| + | * Left in 37 degree incubator for incubation till next morning | ||
| + | |||
| + | === Comments: === | ||
| + | None | ||
Latest revision as of 23:05, 26 June 2012
June 05, 2012: 09:30
Contents |
Observations of Plates
- Done after spreading transformed cells on LB+Amp plates overnight
| Name | #Colonies: Plate 1 | #Colonies: Plate 2 |
|---|---|---|
| Charlie | 1 | 0 |
| dNP138 | 7 | 3 |
| - Control | 0 | 0 |
| + Control | TMTC (>200) | TMTC (>200) |
- Note: Charlie only had 1 colony, photo was taken after picking up colony
- Taken using Red Gel Documentation System from Westburg
Comments:
Successful transformation, negative control has none, positive control has TMTC. However, plasmid concentrations/purity are significantly lower than expected.
June 05, 2012: 16:00
Inoculation of cells
- Took 1 colony of cells from DNP and Charlie respectively and inoculated in 3 ml of LB+Amp media
- Left in 37 degree incubator for incubation till next morning
Comments:
None
