Team:Carnegie Mellon/Notebook/5 June 2012

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'''June 05, 2012:'''
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* Observations of Plates: 9:30am
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'''June 05, 2012: 09:30 '''  
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** Done after spreading transformed cells on LB+Amp plates overnight
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== Observations of Plates ==
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* Done after spreading transformed cells on LB+Amp plates overnight
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{| class="wikitable" border=1 align=left style="text-align: center;"
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{| class="wikitable" style="text-align: center; float:right;" align="center"
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|+ Plate Observations
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! Name !! #Colonies: Plate 1 !! #Colonies: Plate 2
! Name !! #Colonies: Plate 1 !! #Colonies: Plate 2
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<br/><br/><br/><br/><br/><br/><br/><br/><br/>
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[[File:Carnegie_05_June_2012_im1.jpg | LB+Amp Control Groups | border | 500px]]
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<br/><br/>
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[[File:Carnegie_05_June_2012_im2.jpg | LB+Amp Charlie Group | border | 500px]]
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* Note: Charlie only had 1 colony, photo was taken after picking up colony
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* Taken using Red Gel Documentation System from Westburg
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{| class="wikitable" style="text-align: center;" align:"center"
 
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! Name !! #Colonies: Plate 1 !! #Colonies: Plate 2
 
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| Charlie || 1 || 0
 
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| dNP138 || 7 || 3
 
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| <nowiki/>- Control || 0 || 0
 
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| <nowiki/>+ Control || TMTC (>200) || TMTC (>200)
 
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|}
 
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=== Comments: ===
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Successful transformation, negative control has none, positive control has TMTC. However, plasmid concentrations/purity are significantly lower than expected.
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'''June 05, 2012: 16:00 '''
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== Inoculation of cells ==
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* Took 1 colony of cells from DNP and Charlie respectively and inoculated in 3 ml of LB+Amp media
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* Left in 37 degree incubator for incubation till next morning
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{| border=1 align=center
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=== Comments: ===
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None
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! Name !! #Colonies: Plate 1 !! #Colonies: Plate 2
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| Charlie || 1 || 0
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| dNP138 || 7 || 3
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| <nowiki/>- Control || 0 || 0
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| <nowiki/>+ Control || TMTC (>200) || TMTC (>200)
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|}
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Latest revision as of 23:05, 26 June 2012

June 05, 2012: 09:30

Contents

Observations of Plates

  • Done after spreading transformed cells on LB+Amp plates overnight
Plate Observations
Name #Colonies: Plate 1 #Colonies: Plate 2
Charlie 1 0
dNP138 7 3
- Control 0 0
+ Control TMTC (>200) TMTC (>200)










LB+Amp Control Groups

LB+Amp Charlie Group

  • Note: Charlie only had 1 colony, photo was taken after picking up colony
  • Taken using Red Gel Documentation System from Westburg


Comments:

Successful transformation, negative control has none, positive control has TMTC. However, plasmid concentrations/purity are significantly lower than expected.


June 05, 2012: 16:00

Inoculation of cells

  • Took 1 colony of cells from DNP and Charlie respectively and inoculated in 3 ml of LB+Amp media
  • Left in 37 degree incubator for incubation till next morning

Comments:

None

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