Team:Carnegie Mellon/Notebook/4 June 2012

From 2012.igem.org

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* Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively.
* Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively.
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** Harvested DH5-alpha cells from stock culture and made cells competent.
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* Harvested DH5-alpha cells from stock culture and made cells competent.
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*** Followed transformation protocol from Dr. Doonan’s lab (see Protocols)
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* Followed transformation protocol from Dr. Doonan’s lab (see Protocols)

Revision as of 20:59, 26 June 2012

6/04

  • Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively.
  • Harvested DH5-alpha cells from stock culture and made cells competent.
  • Followed transformation protocol from Dr. Doonan’s lab (see Protocols)