Team:Carnegie Mellon/Notebook/4 June 2012

From 2012.igem.org

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'''6/04'''
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** Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively.
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* Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively.
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** Harvested DH5-alpha cells from stock culture and made cells competent.
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* Harvested DH5-alpha cells from stock culture and made cells competent.
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** Followed transformation protocol from Dr. Doonan’s lab (see Protocols)
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* Followed transformation protocol from Dr. Doonan’s lab (see Protocols)
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Revision as of 21:00, 26 June 2012

  • 6/04
    • Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively.
    • Harvested DH5-alpha cells from stock culture and made cells competent.
    • Followed transformation protocol from Dr. Doonan’s lab (see Protocols)