http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Results&feed=atom&action=history
Team:Carnegie Mellon/Met-Results - Revision history
2024-03-29T06:45:58Z
Revision history for this page on the wiki
MediaWiki 1.16.0
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Results&diff=297617&oldid=prev
Ychoo at 03:30, 27 October 2012
2012-10-27T03:30:47Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:30, 27 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 3:</td>
<td colspan="2" class="diff-lineno">Line 3:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- Nav Bar --></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- Nav Bar --></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul class="sf-menu sf-navbar"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul class="sf-menu sf-navbar"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li style ='width: 193px'></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li style ='width: 193px'></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 16:</td>
<td colspan="2" class="diff-lineno">Line 15:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Hom-Team">Team</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Hom-Team">Team</a></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Hom-Attributions">Attributions</a></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 72:</td>
<td colspan="2" class="diff-lineno">Line 74:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Mod-Matlab">Matlab</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Mod-Matlab">Matlab</a></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Mod-Expanded">Expanded</a></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul> </div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 102:</td>
<td colspan="2" class="diff-lineno">Line 107:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br /><br /><br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br /><br /><br /></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>
Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Results&diff=292263&oldid=prev
Enpederson at 23:44, 26 October 2012
2012-10-26T23:44:11Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:44, 26 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 155:</td>
<td colspan="2" class="diff-lineno">Line 155:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://igem.org/<del class="diffchange diffchange-inline">Image:</del>CMU_Spin-DFHBI2.jpg", width="689", height="384"><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="https://<ins class="diffchange diffchange-inline">static.</ins>igem.org/<ins class="diffchange diffchange-inline">mediawiki/igem.org/5/5c/</ins>CMU_Spin-DFHBI2.jpg", width="689", height="384"><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Figure 3: Spinach fluorescence (reporter for RNA levels) over time</strong>. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Figure 3: Spinach fluorescence (reporter for RNA levels) over time</strong>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
</table>
Enpederson
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Results&diff=287002&oldid=prev
Enpederson at 17:24, 26 October 2012
2012-10-26T17:24:15Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 17:24, 26 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 167:</td>
<td colspan="2" class="diff-lineno">Line 167:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="http://partsregistry.org/wiki/images/0/09/CMU_leakyp.jpg", width="<del class="diffchange diffchange-inline">465</del>", height="<del class="diffchange diffchange-inline">594</del>"><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="http://partsregistry.org/wiki/images/0/09/CMU_leakyp.jpg", width="<ins class="diffchange diffchange-inline">464</ins>", height="<ins class="diffchange diffchange-inline">295</ins>"><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="http://partsregistry.org/wiki/images/b/b4/CMU_leakyr.jpg", width="465", height="<del class="diffchange diffchange-inline">594</del>"><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="http://partsregistry.org/wiki/images/b/b4/CMU_leakyr.jpg", width="465", height="<ins class="diffchange diffchange-inline">295</ins>"><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><strong>Figure 5: Leaky RNA (top panel) and protein (bottom panel) expression levels of our T7Lac promoters in BL21(DE3) cells.</strong> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><strong>Figure 5: Leaky RNA (top panel) and protein (bottom panel) expression levels of our T7Lac promoters in BL21(DE3) cells.</strong> </div></td></tr>
</table>
Enpederson
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Results&diff=286990&oldid=prev
Enpederson at 17:23, 26 October 2012
2012-10-26T17:23:36Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 17:23, 26 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 167:</td>
<td colspan="2" class="diff-lineno">Line 167:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="http://partsregistry.org/wiki/images/<del class="diffchange diffchange-inline">2</del>/<del class="diffchange diffchange-inline">25</del>/<del class="diffchange diffchange-inline">CMU_leaky</del>.jpg", width="465", height="594"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="http://partsregistry.org/wiki/images/<ins class="diffchange diffchange-inline">0</ins>/<ins class="diffchange diffchange-inline">09</ins>/<ins class="diffchange diffchange-inline">CMU_leakyp</ins>.jpg", width="465", height="594"><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><img src="http://partsregistry.org/wiki/images/b/b4/CMU_leakyr.jpg", width="465", height="594"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><strong>Figure 5: Leaky RNA (top panel) and protein (bottom panel) expression levels of our T7Lac promoters in BL21(DE3) cells.</strong> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><strong>Figure 5: Leaky RNA (top panel) and protein (bottom panel) expression levels of our T7Lac promoters in BL21(DE3) cells.</strong> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
</table>
Enpederson
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Results&diff=286930&oldid=prev
Enpederson at 17:20, 26 October 2012
2012-10-26T17:20:13Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 17:20, 26 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 155:</td>
<td colspan="2" class="diff-lineno">Line 155:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="<del class="diffchange diffchange-inline">http</del>://<del class="diffchange diffchange-inline">partsregistry</del>.org/<del class="diffchange diffchange-inline">wiki/images/5/5c/</del>CMU_Spin-DFHBI2.jpg", width="689", height="384"><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="<ins class="diffchange diffchange-inline">https</ins>://<ins class="diffchange diffchange-inline">igem</ins>.org/<ins class="diffchange diffchange-inline">Image:</ins>CMU_Spin-DFHBI2.jpg", width="689", height="384"><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Figure 3: Spinach fluorescence (reporter for RNA levels) over time</strong>. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Figure 3: Spinach fluorescence (reporter for RNA levels) over time</strong>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 161:</td>
<td colspan="2" class="diff-lineno">Line 161:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="<del class="diffchange diffchange-inline">http</del>://<del class="diffchange diffchange-inline">partsregistry</del>.org/<del class="diffchange diffchange-inline">wiki</del>/<del class="diffchange diffchange-inline">images</del>/d/d0/CMU_FAP-MG2.jpg", width="689", height="384"><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="<ins class="diffchange diffchange-inline">https</ins>://<ins class="diffchange diffchange-inline">static.igem</ins>.org/<ins class="diffchange diffchange-inline">mediawiki</ins>/<ins class="diffchange diffchange-inline">igem.org</ins>/d/d0/CMU_FAP-MG2.jpg", width="689", height="384"><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Figure 4: FAP fluorescence (reporter for protein levels) over time </strong>.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Figure 4: FAP fluorescence (reporter for protein levels) over time </strong>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
</table>
Enpederson
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Results&diff=285786&oldid=prev
Enpederson at 15:49, 26 October 2012
2012-10-26T15:49:37Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 15:49, 26 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 129:</td>
<td colspan="2" class="diff-lineno">Line 129:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Dosage Curves of Spinach and FAP with Their Respective Dyes</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Dosage Curves of Spinach and FAP with Their Respective Dyes</h1></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To understand binding kinetics of both Spinach and FAP with their respective fluorogens, we collected dosage curves of the biosensors by adding different concentrations of fluorogens to bacteria that express both Spinach and FAP. Based on the dosage curves, we calculated dissociation constants (K<sub>D</sub>) of each biosensor-fluorogen complex and saturating dose of each fluorogen. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To understand binding kinetics <ins class="diffchange diffchange-inline">and equilibria </ins>of both Spinach and FAP with their respective fluorogens, we collected dosage curves of the biosensors by adding different concentrations of fluorogens to bacteria that express both Spinach and FAP. Based on the dosage curves, we calculated dissociation constants (K<sub>D</sub>) of each biosensor-fluorogen complex and saturating dose of each fluorogen. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 138:</td>
<td colspan="2" class="diff-lineno">Line 138:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The measured K<sub>D</sub> of the Spinach-DFHBI complex is 537nM <sup>[2]</sup>. Our measured K<sub>D</sub> <del class="diffchange diffchange-inline">is 100 times smaller than the published K<sub>D</sub>. We hypothesized that this could be due to magnesium concentration inside bacteria because it </del>has <del class="diffchange diffchange-inline">been shown that the binding of DFHBI by Spinach is sensitive to magnesium concentration</del>. We also measured the dosage curves at both 10th and 60th minute timepoint. We did not observe significant differences in the fluorescence levels, suggesting that DFHBI diffusion across bacterial membrane and its binding to Spinach occurs rapidly. Lines are drawn as guide of eyes. Please refer to the Protocols page for details of experiments. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The measured K<sub>D</sub> of the Spinach-DFHBI complex is 537nM <sup>[2]</sup>. Our measured K<sub>D</sub> <ins class="diffchange diffchange-inline">also </ins>has <ins class="diffchange diffchange-inline"> a nanomolar affinity</ins>. <ins class="diffchange diffchange-inline"> </ins>We also measured the dosage curves at both 10th and 60th minute timepoint. We did not observe significant differences in the fluorescence levels, suggesting that DFHBI diffusion across bacterial membrane and its binding to Spinach occurs rapidly. Lines are drawn as guide of eyes. Please refer to the Protocols page for details of experiments. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>
Enpederson
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Results&diff=264527&oldid=prev
Ychoo at 01:57, 4 October 2012
2012-10-04T01:57:11Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:57, 4 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 171:</td>
<td colspan="2" class="diff-lineno">Line 171:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The leaky expression level of BBa_K921000 promoter is noticeably lower than the wild type promoter, which is consistent with the low RNA expression rates after induction of the promoter. However, the leaky expression levels of both BBa_K921001 and BBa_K921002 promoters are noticeably higher than the wild type promoter. Uninduced cells (without IPTG) were added to wells in a 96 well plate supplemented with either 200µM of DFHBI or 10µM of malachite green. Fluorescence intensities at the 3rd hour time point are shown for comparison between promoters.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The leaky expression level of BBa_K921000 promoter is noticeably lower than the wild type promoter, which is consistent with the low RNA expression rates after induction of the promoter. However, the leaky expression levels of both BBa_K921001 and BBa_K921002 promoters are noticeably higher than the wild type promoter. Uninduced cells (without IPTG) were added to wells in a 96 well plate supplemented with either 200µM of DFHBI or 10µM of malachite green. Fluorescence intensities at the 3rd hour time point are shown for comparison between promoters.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
</table>
Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Results&diff=264514&oldid=prev
Ychoo at 01:56, 4 October 2012
2012-10-04T01:56:52Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:56, 4 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 176:</td>
<td colspan="2" class="diff-lineno">Line 176:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2012/d/de/Ts.png" height="300" width="380" align="center"/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2012/d/de/Ts.png" height="300" width="380" align="center"/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2012/9/9b/Tl.png" height="300" width="380" ><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2012/9/9b/Tl.png" height="300" width="380" ><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><strong>Figure <del class="diffchange diffchange-inline">4</del>: Calculated values of transcription strength and translation efficiency.</strong></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><strong>Figure <ins class="diffchange diffchange-inline">6</ins>: Calculated values of transcription strength <ins class="diffchange diffchange-inline">(left panel) </ins>and translation efficiency <ins class="diffchange diffchange-inline">(right panel)</ins>.</strong></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">These values were calculated from the raw </del>fluorescence values <del class="diffchange diffchange-inline">that </del>were normalized by OD600 <del class="diffchange diffchange-inline">and fit into </del>the differential equations that <del class="diffchange diffchange-inline">were </del>developed to model our system<del class="diffchange diffchange-inline">. The units are in RFU/min due to time constraints and we were not able to develop a conversion factor from fluorescence intensity to concentration. Due to the dye-binding nature of our system, however, it is theoretically possible to develop an assay to determine concentrations. Keep in mind that fluorescence intensities directly relate to the amount of dye bound to the FAP/aptamer</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Raw </ins>fluorescence values were normalized by OD600<ins class="diffchange diffchange-inline">. Next, </ins>the <ins class="diffchange diffchange-inline">normalized fluorescence values were fitted using </ins>differential equations that <ins class="diffchange diffchange-inline">we </ins>developed to model our system. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
</table>
Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Results&diff=263898&oldid=prev
Ychoo at 01:31, 4 October 2012
2012-10-04T01:31:13Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:31, 4 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 79:</td>
<td colspan="2" class="diff-lineno">Line 79:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Overview">Human Practices</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Overview">Human Practices</a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li class = 'offset' style ='width: <del class="diffchange diffchange-inline">622px</del>'> <a href="#"></a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li class = 'offset' style ='width: <ins class="diffchange diffchange-inline">302px</ins>'> <a href="#"></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Overview">Overview</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Overview">Overview</a></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 91:</td>
<td colspan="2" class="diff-lineno">Line 91:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Software">Software</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Software">Software</a></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Team">Team Presentation</a></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Teaching">Teaching Presentation</a></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul> </div></td></tr>
</table>
Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Results&diff=263309&oldid=prev
Enpederson at 01:04, 4 October 2012
2012-10-04T01:04:11Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:04, 4 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 165:</td>
<td colspan="2" class="diff-lineno">Line 165:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The leaky expression level of BBa_K921000 promoter is noticeably lower than the wild type promoter, which is consistent with the low RNA expression rates after induction of the promoter. However, the leaky expression levels of both BBa_K921001 and BBa_K921002 promoters are noticeably higher than the wild type promoter. Uninduced cells (without IPTG) were added to wells in a 96 well plate supplemented with either 200µM of DFHBI or 10µM of malachite green. Fluorescence intensities at the 3rd hour time point are shown for comparison between promoters.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The leaky expression level of BBa_K921000 promoter is noticeably lower than the wild type promoter, which is consistent with the low RNA expression rates after induction of the promoter. However, the leaky expression levels of both BBa_K921001 and BBa_K921002 promoters are noticeably higher than the wild type promoter. Uninduced cells (without IPTG) were added to wells in a 96 well plate supplemented with either 200µM of DFHBI or 10µM of malachite green. Fluorescence intensities at the 3rd hour time point are shown for comparison between promoters.</p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><img src="https://static.igem.org/mediawiki/2012/d/de/Ts.png" height="300" width="380" align="center"/></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><img src="https://static.igem.org/mediawiki/2012/9/9b/Tl.png" height="300" width="380" ><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><strong>Figure 4: Calculated values of transcription strength and translation efficiency.</strong></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">These values were calculated from the raw fluorescence values that were normalized by OD600 and fit into the differential equations that were developed to model our system. The units are in RFU/min due to time constraints and we were not able to develop a conversion factor from fluorescence intensity to concentration. Due to the dye-binding nature of our system, however, it is theoretically possible to develop an assay to determine concentrations. Keep in mind that fluorescence intensities directly relate to the amount of dye bound to the FAP/aptamer. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
</table>
Enpederson