http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Notebook&feed=atom&action=history
Team:Carnegie Mellon/Met-Notebook - Revision history
2024-03-29T13:37:52Z
Revision history for this page on the wiki
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http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Notebook&diff=297677&oldid=prev
Ychoo at 03:32, 27 October 2012
2012-10-27T03:32:14Z
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Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Notebook&diff=263927&oldid=prev
Ychoo at 01:32, 4 October 2012
2012-10-04T01:32:20Z
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Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Notebook&diff=262241&oldid=prev
Ychoo at 00:12, 4 October 2012
2012-10-04T00:12:59Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. <a href="https://docs.google.com/document/d/1rLIBV3wwV6zzKGaTIbFQf2UdksK0lluRZFd-njcyLqk/edit">Week 3</a> was focused transforming two different FAP plasmids we obtained into DH5a and working out the exact construct design </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. <a href="https://docs.google.com/document/d/1rLIBV3wwV6zzKGaTIbFQf2UdksK0lluRZFd-njcyLqk/edit">Week 3</a> was focused transforming two different FAP plasmids we obtained into DH5a and working out the exact construct design </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br \></ins><br \></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. <a href="https://docs.google.com/document/d/1Hf4BhpGf1OCukNzULbElX0ZgVzSmGa7Fu4kH8AxSxE8/edit">Week 4</a> was focused on testing and comparing the functionality of the two FAP plasmids expressed in BL21DE3 cells using two variants of Malachite Green (5% EtOH and PBS)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. <a href="https://docs.google.com/document/d/1Hf4BhpGf1OCukNzULbElX0ZgVzSmGa7Fu4kH8AxSxE8/edit">Week 4</a> was focused on testing and comparing the functionality of the two FAP plasmids expressed in BL21DE3 cells using two variants of Malachite Green (5% EtOH and PBS)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br \></ins><br \></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3. <a href="https://docs.google.com/document/d/1r4RECG92rop1xuOs-0jIC303CcVzAKN3bNZLa1iaxvw/edit">Week 5</a> was focused on cloning our synthesized Spinach cassette into our vector, and conducting dosage and temporal curves using the FAP and MG(5% EtOH) used.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3. <a href="https://docs.google.com/document/d/1r4RECG92rop1xuOs-0jIC303CcVzAKN3bNZLa1iaxvw/edit">Week 5</a> was focused on cloning our synthesized Spinach cassette into our vector, and conducting dosage and temporal curves using the FAP and MG(5% EtOH) used.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br \></ins><br \></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4. <a href="https://docs.google.com/document/d/1wH9gUvaaxaLmb3aetGs9DwUoSB7h6yoLNuoviSy1w2A/edit">Week 6</a> was focused on Spinach. We ran a gel and sequenced our Spinach transformant and also PCR amplified the original cassette to obtain more copies.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4. <a href="https://docs.google.com/document/d/1wH9gUvaaxaLmb3aetGs9DwUoSB7h6yoLNuoviSy1w2A/edit">Week 6</a> was focused on Spinach. We ran a gel and sequenced our Spinach transformant and also PCR amplified the original cassette to obtain more copies.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br \></ins><br \></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>5. <a href="https://docs.google.com/document/d/1AoaOuvT6I6cKguLkRqwm-l32u_pYSiKQAg8qbecLgf8/edit">Week 7</a> was focused on Spinach cloning. We tried another round of cloning of Spinach into the FAP vector and a pET vector. Also provides the basis for the first T7 promoter designs. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>5. <a href="https://docs.google.com/document/d/1AoaOuvT6I6cKguLkRqwm-l32u_pYSiKQAg8qbecLgf8/edit">Week 7</a> was focused on Spinach cloning. We tried another round of cloning of Spinach into the FAP vector and a pET vector. Also provides the basis for the first T7 promoter designs. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br \></ins><br \></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>6. <a href="https://docs.google.com/document/d/1Xi6JxTts2XbQGFtpnM92sDUSm4DftFqjyUGsjhRcQ2o/edit">Week 8</a> was focused again on cloning Spinach into the FAP vector and the pET vector after another negative result of cloning. Finalized the T7 promoter designs</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>6. <a href="https://docs.google.com/document/d/1Xi6JxTts2XbQGFtpnM92sDUSm4DftFqjyUGsjhRcQ2o/edit">Week 8</a> was focused again on cloning Spinach into the FAP vector and the pET vector after another negative result of cloning. Finalized the T7 promoter designs</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br \></ins><br \></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>7. <a href="https://docs.google.com/document/d/1nYWf_uWMG4JdDJ7qKhkv9az2DsZQ-70-uqPGlUS5Jt4/edit">Week 9</a> was focused on characterizing the Spinach cassette in a pET vector. Efforts continued to create a clone with both the FAP and the Spinach Cassettes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>7. <a href="https://docs.google.com/document/d/1nYWf_uWMG4JdDJ7qKhkv9az2DsZQ-70-uqPGlUS5Jt4/edit">Week 9</a> was focused on characterizing the Spinach cassette in a pET vector. Efforts continued to create a clone with both the FAP and the Spinach Cassettes.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br \></ins><br \></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>8. <a href="https://docs.google.com/document/d/1zschOOFerRaZeCdbADdh4lld2_oindBloPdJZD3DPXs/edit">Week 10</a> focused on cloning Spinach and the FAP into a different vector with entirely new restriction sites. Began preparation for a cloning round in URA- yeast as a backup plan. Cloned cassettes into pIVEX vector.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>8. <a href="https://docs.google.com/document/d/1zschOOFerRaZeCdbADdh4lld2_oindBloPdJZD3DPXs/edit">Week 10</a> focused on cloning Spinach and the FAP into a different vector with entirely new restriction sites. Began preparation for a cloning round in URA- yeast as a backup plan. Cloned cassettes into pIVEX vector.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br \></ins><br \></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>9. <a href="https://docs.google.com/document/d/1Oe9OEPeRk6LF2vfuey5QqRgAWYewko9sDssDii5KTds/edit">Week 11</a> included cloning round in URA- yeast using homologous recombination. Characterized Spinach using a functional assay in pIVEX vector.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>9. <a href="https://docs.google.com/document/d/1Oe9OEPeRk6LF2vfuey5QqRgAWYewko9sDssDii5KTds/edit">Week 11</a> included cloning round in URA- yeast using homologous recombination. Characterized Spinach using a functional assay in pIVEX vector.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br \></ins><br \></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>10. <a href="https://docs.google.com/document/d/1f8m0_xXKlDkqEjsDUJNTVHA0Cohd76ylFR3BXms4Td4/edit">Week 12</a> is a brief document detailing the PCR reaction, which is in preparation for the Fall laboratory rounds or cloning.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>10. <a href="https://docs.google.com/document/d/1f8m0_xXKlDkqEjsDUJNTVHA0Cohd76ylFR3BXms4Td4/edit">Week 12</a> is a brief document detailing the PCR reaction, which is in preparation for the Fall laboratory rounds or cloning.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>11. <a href="https://docs.google.com/document/d/1q4LFNBFHz5GOIxMp1fbecpJ3SVy9t7eWDwCCFCMD7HU/edit">Fall notebook </a> is a consolidated log of all our experiments carried through Fall, including the final characterization and cloning experiments.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>11. <a href="https://docs.google.com/document/d/1q4LFNBFHz5GOIxMp1fbecpJ3SVy9t7eWDwCCFCMD7HU/edit">Fall notebook </a> is a consolidated log of all our experiments carried through Fall, including the final characterization and cloning experiments.</div></td></tr>
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Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Notebook&diff=250303&oldid=prev
Ychoo at 14:08, 2 October 2012
2012-10-02T14:08:14Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Bio-Submitted">Submitted Parts</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Bio-Submitted">Submitted Parts</a></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Met-Notebook">Notebook</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Met-Notebook">Notebook</a></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </li></ins></div></td></tr>
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Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Notebook&diff=245975&oldid=prev
Ychoo at 05:29, 1 October 2012
2012-10-01T05:29:51Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 05:29, 1 October 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br \></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>10. <a href="https://docs.google.com/document/d/1f8m0_xXKlDkqEjsDUJNTVHA0Cohd76ylFR3BXms4Td4/edit">Week 12</a> is a brief document detailing the PCR reaction, which is in preparation for the Fall laboratory rounds or cloning.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>10. <a href="https://docs.google.com/document/d/1f8m0_xXKlDkqEjsDUJNTVHA0Cohd76ylFR3BXms4Td4/edit">Week 12</a> is a brief document detailing the PCR reaction, which is in preparation for the Fall laboratory rounds or cloning.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br \></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">11. <a href="https://docs.google.com/document/d/1q4LFNBFHz5GOIxMp1fbecpJ3SVy9t7eWDwCCFCMD7HU/edit">Fall notebook </a> is a consolidated log of all our experiments carried through Fall, including the final characterization and cloning experiments.</ins></div></td></tr>
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Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Notebook&diff=245968&oldid=prev
Ychoo at 05:27, 1 October 2012
2012-10-01T05:27:30Z
<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h1 id = "section1-2"> <del class="diffchange diffchange-inline">Links to </del>Notebooks</h1></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h1 id = "section1-2"> Notebooks</h1></div></td></tr>
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Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Notebook&diff=245859&oldid=prev
Ychoo at 04:48, 1 October 2012
2012-10-01T04:48:38Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>During the first 2 weeks after final exams, we were mainly focused on settling the logistics of getting the project off the ground. Hence there were no lab experiments and hence no notebook kept. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>During the first 2 weeks after final exams, we were mainly focused on settling the logistics of getting the project off the ground. Hence there were no lab experiments and hence no notebook kept. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br \></ins><br \></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. <a href="https://docs.google.com/document/d/1rLIBV3wwV6zzKGaTIbFQf2UdksK0lluRZFd-njcyLqk/edit">Week 3</a> was focused transforming two different FAP plasmids we obtained into DH5a and working out the exact construct design </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. <a href="https://docs.google.com/document/d/1rLIBV3wwV6zzKGaTIbFQf2UdksK0lluRZFd-njcyLqk/edit">Week 3</a> was focused transforming two different FAP plasmids we obtained into DH5a and working out the exact construct design </div></td></tr>
</table>
Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Notebook&diff=245858&oldid=prev
Ychoo at 04:48, 1 October 2012
2012-10-01T04:48:11Z
<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><p></del><h1 id = "section 1-1"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h1 id = "section 1-1"> Overview</h1></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><id = "section1-1"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><id = "section1-1"></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lab notebooks were kept using Google docs, and they mostly contain information from the experimental side, including details such as the protocol followed, timestamp of the experiments and the observed results. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lab notebooks were kept using Google docs, and they mostly contain information from the experimental side, including details such as the protocol followed, timestamp of the experiments and the observed results. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br \></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">During the first 2 weeks after final exams, we were mainly focused on settling the logistics of getting the project off the ground. Hence there were no lab experiments and hence no notebook kept. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br \></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Feel free to click on the links below to view the actual lab notebooks and email us if you have any questions!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Feel free to click on the links below to view the actual lab notebooks and email us if you have any questions!</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>Links to Notebooks</h1></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">During the first 2 weeks after final exams, we were mainly focused on settling the logistics of getting the project off the ground. Hence there were no lab experiments and hence no notebook kept. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br \</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. <a href="https://docs.google.com/document/d/1rLIBV3wwV6zzKGaTIbFQf2UdksK0lluRZFd-njcyLqk/edit">Week 3</a> was focused transforming two different FAP plasmids we obtained into DH5a and working out the exact construct design </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. <a href="https://docs.google.com/document/d/1rLIBV3wwV6zzKGaTIbFQf2UdksK0lluRZFd-njcyLqk/edit">Week 3</a> was focused transforming two different FAP plasmids we obtained into DH5a and working out the exact construct design </div></td></tr>
</table>
Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Notebook&diff=245857&oldid=prev
Ychoo at 04:47, 1 October 2012
2012-10-01T04:47:00Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 04:47, 1 October 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="#" class="toc-link" id="toc-link"><span>&#9660;</span> Table of Contents</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="#" class="toc-link" id="toc-link"><span>&#9660;</span> Table of Contents</a></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">h1 id = "section1"</del>><del class="diffchange diffchange-inline">Notebook</del><<del class="diffchange diffchange-inline">/</del>h1<del class="diffchange diffchange-inline">></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">p</ins>><h1 id = "section 1-1"></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lab notebooks were kept using Google docs, and they mostly contain information from the experimental side, including details such as the protocol followed, timestamp of the experiments and the observed results. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lab notebooks were kept using Google docs, and they mostly contain information from the experimental side, including details such as the protocol followed, timestamp of the experiments and the observed results. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><b> </del>Links to Notebooks</<del class="diffchange diffchange-inline">b></h2</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>Links to Notebooks</<ins class="diffchange diffchange-inline">h1</ins>></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. <a href="https://docs.google.com/document/d/1rLIBV3wwV6zzKGaTIbFQf2UdksK0lluRZFd-njcyLqk/edit">Week 3</a> was focused transforming two different FAP plasmids we obtained into DH5a and working out the exact construct design </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. <a href="https://docs.google.com/document/d/1rLIBV3wwV6zzKGaTIbFQf2UdksK0lluRZFd-njcyLqk/edit">Week 3</a> was focused transforming two different FAP plasmids we obtained into DH5a and working out the exact construct design </div></td></tr>
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Ychoo
http://2012.igem.org/wiki/index.php?title=Team:Carnegie_Mellon/Met-Notebook&diff=207218&oldid=prev
Enpederson at 17:44, 26 September 2012
2012-09-26T17:44:02Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:44, 26 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4. <a href="https://docs.google.com/document/d/1wH9gUvaaxaLmb3aetGs9DwUoSB7h6yoLNuoviSy1w2A/edit">Week 6</a> was focused on Spinach. We ran a gel and sequenced our Spinach transformant and also PCR amplified the original cassette to obtain more copies.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4. <a href="https://docs.google.com/document/d/1wH9gUvaaxaLmb3aetGs9DwUoSB7h6yoLNuoviSy1w2A/edit">Week 6</a> was focused on Spinach. We ran a gel and sequenced our Spinach transformant and also PCR amplified the original cassette to obtain more copies.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br \></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>5. <a href="https://docs.google.com/document/d/1AoaOuvT6I6cKguLkRqwm-l32u_pYSiKQAg8qbecLgf8/edit">Week 7</a> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>5. <a href="https://docs.google.com/document/d/1AoaOuvT6I6cKguLkRqwm-l32u_pYSiKQAg8qbecLgf8/edit">Week 7</a> <ins class="diffchange diffchange-inline">was focused on Spinach cloning. We tried another round of cloning of Spinach into the FAP vector and a pET vector. Also provides the basis for the first T7 promoter designs. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br \></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>6. <a href="https://docs.google.com/document/d/1Xi6JxTts2XbQGFtpnM92sDUSm4DftFqjyUGsjhRcQ2o/edit">Week 8</a> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>6. <a href="https://docs.google.com/document/d/1Xi6JxTts2XbQGFtpnM92sDUSm4DftFqjyUGsjhRcQ2o/edit">Week 8</a> <ins class="diffchange diffchange-inline">was focused again on cloning Spinach into the FAP vector and the pET vector after another negative result of cloning. Finalized the T7 promoter designs</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br \></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>7. <a href="https://docs.google.com/document/d/1nYWf_uWMG4JdDJ7qKhkv9az2DsZQ-70-uqPGlUS5Jt4/edit">Week 9</a> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>7. <a href="https://docs.google.com/document/d/1nYWf_uWMG4JdDJ7qKhkv9az2DsZQ-70-uqPGlUS5Jt4/edit">Week 9</a> <ins class="diffchange diffchange-inline">was focused on characterizing the Spinach cassette in a pET vector. Efforts continued to create a clone with both the FAP and the Spinach Cassettes.</ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>8. <a href="https://docs.google.com/document/d/1zschOOFerRaZeCdbADdh4lld2_oindBloPdJZD3DPXs/edit">Week 10</a> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>8. <a href="https://docs.google.com/document/d/1zschOOFerRaZeCdbADdh4lld2_oindBloPdJZD3DPXs/edit">Week 10</a> <ins class="diffchange diffchange-inline">focused on cloning Spinach and the FAP into a different vector with entirely new restriction sites. Began preparation for a cloning round in URA- yeast as a backup plan. Cloned cassettes into pIVEX vector.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br \></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br \></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>9. <a href="https://docs.google.com/document/d/1Oe9OEPeRk6LF2vfuey5QqRgAWYewko9sDssDii5KTds/edit">Week 11</a> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>9. <a href="https://docs.google.com/document/d/1Oe9OEPeRk6LF2vfuey5QqRgAWYewko9sDssDii5KTds/edit">Week 11</a> <ins class="diffchange diffchange-inline">included cloning round in URA- yeast using homologous recombination. Characterized Spinach using a functional assay in pIVEX vector.</ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>10. <a href="https://docs.google.com/document/d/1f8m0_xXKlDkqEjsDUJNTVHA0Cohd76ylFR3BXms4Td4/edit">Week 12</a> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>10. <a href="https://docs.google.com/document/d/1f8m0_xXKlDkqEjsDUJNTVHA0Cohd76ylFR3BXms4Td4/edit">Week 12</a> <ins class="diffchange diffchange-inline">is a brief document detailing the PCR reaction, which is in preparation for the Fall laboratory rounds or cloning.</ins></div></td></tr>
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Enpederson