Team:Carnegie Mellon/Bio-Submitted

From 2012.igem.org

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RBS used in construct is: CATATG <b>AAGAAGGAGA</b> TATACC
RBS used in construct is: CATATG <b>AAGAAGGAGA</b> TATACC
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Using the model we developed in MATLAB, we calculated the following properties with respect to the wild-type promoter.
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<table border="1">
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<tr>
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<th>Promoter</th>
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<td> Mutant I </td>
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<td> Mutant II </td>
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<td> Mutant III </td>
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</tr>
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<tr>
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<th>Transcription Strength</th>
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<td> 97% </td>
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<td> 72% </td>
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<td> 127% </td>
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</tr>
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<tr>
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<th>Translational Efficiency</th>
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<td> 6%</td>
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<td> 6%</td>
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<td> 94%</td>
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</tr>
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<tr>
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<th>RNA degradation constant (assumed)</th>
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<td> 100% </td>
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<td> 100% </td>
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<td> 100% </td>
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</tr>
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<tr>
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<th>Protein degradation constant (fit)</th>
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<td> 4% </td>
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<td> 6% </td>
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<td> 60% </td>
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</tr>
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</table>
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</html>
</html>
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Revision as of 21:10, 1 October 2012

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Submitted Parts

We have submitted three T7Lac promoter parts to the registry. The followings show the sequences of these constructs. BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA
Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA
Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA

Predicted strength of the hybrid T7Lac promoters

Expected promoter strength of the mutants (relative to BBa_K613007):
Mutant I: <100%
Mutant II: ~100%
Mutant III: ~50%

Expected LacI leaky expression of different mutants:
Mutant I: More than average
Mutant II: Average
Mutant III: Average

RBS used in construct is: CATATG AAGAAGGAGA TATACC Using the model we developed in MATLAB, we calculated the following properties with respect to the wild-type promoter.
Promoter Mutant I Mutant II Mutant III
Transcription Strength 97% 72% 127%
Translational Efficiency 6% 6% 94%
RNA degradation constant (assumed) 100% 100% 100%
Protein degradation constant (fit) 4% 6% 60%

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