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Team:Carnegie Mellon/Bio-Submitted
From 2012.igem.org
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<h3 class="pre-experiment">Predicted strength of the hybrid T7Lac promoters</h3> | <h3 class="pre-experiment">Predicted strength of the hybrid T7Lac promoters</h3> | ||
| - | Expected promoter strength of the mutants ( | + | Expected promoter strength of the mutants (relative to BBa_K613007):<br> |
Mutant I: <100% <br> | Mutant I: <100% <br> | ||
Mutant II: ~100% <br> | Mutant II: ~100% <br> | ||
Mutant III: ~50%<br> | Mutant III: ~50%<br> | ||
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</p><p class="leaky"> | </p><p class="leaky"> | ||
Expected LacI leaky expression of different mutants: <br> | Expected LacI leaky expression of different mutants: <br> | ||
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RBS used in construct is: CATATG <b>AAGAAGGAGA</b> TATACC | RBS used in construct is: CATATG <b>AAGAAGGAGA</b> TATACC | ||
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</html> | </html> | ||
{{:Team:Carnegie_Mellon/Templates/Footer}} | {{:Team:Carnegie_Mellon/Templates/Footer}} | ||
Revision as of 21:07, 1 October 2012
Submitted Parts
We have submitted three T7Lac promoter parts to the registry. The followings show the sequences of these constructs.
BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA
Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA
Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA
Predicted strength of the hybrid T7Lac promoters
Expected promoter strength of the mutants (relative to BBa_K613007):Mutant I: <100%
Mutant II: ~100%
Mutant III: ~50%
Expected LacI leaky expression of different mutants:
Mutant I: More than average
Mutant II: Average
Mutant III: Average
