Team:Carnegie Mellon/Bio-Submitted

From 2012.igem.org

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<h1 class="section1"> Submitted Parts </h1>
<h1 class="section1"> Submitted Parts </h1>
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We have submitted three T7Lac promoter parts to the registry. The followings show the sequences of these constructs.
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BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA <br>
BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA <br>
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA <br>
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA <br>
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Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA <br>
Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA <br>
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<h3 class="pre-experiment">Hypothesis:</h3>
 
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Expected promoter strength of different mutants:<br>
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<h3 class="pre-experiment">Predicted strength of the hybrid T7Lac promoters</h3>
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Expected promoter strength of the mutants (%relative strength as compared to the wild type T7Lac promoter BBa_K613007):<br>
Mutant I: <100% <br>
Mutant I: <100% <br>
Mutant II: ~100% <br>
Mutant II: ~100% <br>

Revision as of 21:06, 1 October 2012

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Submitted Parts

We have submitted three T7Lac promoter parts to the registry. The followings show the sequences of these constructs. BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA
Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA
Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA

Predicted strength of the hybrid T7Lac promoters

Expected promoter strength of the mutants (%relative strength as compared to the wild type T7Lac promoter BBa_K613007):
Mutant I: <100%
Mutant II: ~100%
Mutant III: ~50%

Expected LacI leaky expression of different mutants:
Mutant I: More than average
Mutant II: Average
Mutant III: Average

RBS used in construct is: CATATG AAGAAGGAGA TATACC

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