Team:Carnegie Mellon/Bio-Properties

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<a href="https://2012.igem.org/Team:Carnegie_Mellon/Bio-Overview">BioBricks</a>
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<h1>Delete this page: Properties of Promoters</h1>
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BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA <br>
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Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA <br>
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Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA <br>
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Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA <br>
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Using the model we developed in MATLAB, we calculated the following properties with respect to the wild-type promoter.
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<table border="1">
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<th>Promoter</th>
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<td> Mutant I </td>
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<td> Mutant II </td>
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<td> Mutant III </td>
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<th>Transcription Strength</th>
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<td> 97% </td>
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<td> 72% </td>
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<td> 127% </td>
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<th>Translational Efficiency</th>
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<td> 6%</td>
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<td> 6%</td>
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<td> 94%</td>
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<th>RNA degradation constant (assumed)</th>
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<td> 100% </td>
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<td> 100% </td>
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<td> 100% </td>
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<th>Protein degradation constant (fit)</th>
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<td> 4% </td>
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<td> 6% </td>
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<td> 60% </td>
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</table>
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Latest revision as of 21:16, 1 October 2012

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Delete this page: Properties of Promoters

BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA
Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA
Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA

Using the model we developed in MATLAB, we calculated the following properties with respect to the wild-type promoter.
Promoter Mutant I Mutant II Mutant III
Transcription Strength 97% 72% 127%
Translational Efficiency 6% 6% 94%
RNA degradation constant (assumed) 100% 100% 100%
Protein degradation constant (fit) 4% 6% 60%

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