Team:Carnegie Mellon

From 2012.igem.org

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<li class="toclevel-1"><a href="#Introduction:_Motivation"><span class="tocnumber">1</span> <span class="toctext">Introduction: Motivation</span></a></li>
<li class="toclevel-1"><a href="#Introduction:_Motivation"><span class="tocnumber">1</span> <span class="toctext">Introduction: Motivation</span></a></li>
<li class="toclevel-1"><a href="#Primary_Objective:_A_Useful_BioBrick_for_Synthetic_Biologists"><span class="tocnumber">2</span> <span class="toctext">Primary Objective: A Useful BioBrick for Synthetic Biologists</span></a></li>
<li class="toclevel-1"><a href="#Primary_Objective:_A_Useful_BioBrick_for_Synthetic_Biologists"><span class="tocnumber">2</span> <span class="toctext">Primary Objective: A Useful BioBrick for Synthetic Biologists</span></a></li>
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<li class="toclevel-1"><a href="#Secondary_Objective:_Secondary_Objective"><span class="tocnumber">3</span> <span class="toctext">Secondary Objective: Solving vitamin A deficiency in the developing world</span></a></li>
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<li class="toclevel-1"><a href="#Secondary_Objective:_Secondary_Objective"><span class="tocnumber">3</span> <span class="toctext">Secondary Objective: Secondary Objective</span></a></li>
<li class="toclevel-1"><a href="#Further_Considerations"><span class="tocnumber">4</span> <span class="toctext">Further Considerations</span></a></li>
<li class="toclevel-1"><a href="#Further_Considerations"><span class="tocnumber">4</span> <span class="toctext">Further Considerations</span></a></li>
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<p>This will be xxxyyy
<p>This will be xxxyyy
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<a name="Secondary_Objective:_Secondary_Objective"></a><h2> <span class="mw-headline"> Secondary Objective: Solving vitamin A deficiency in the developing world </span></h2>
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<a name="Secondary_Objective:_Secondary_Objective"></a><h2> <span class="mw-headline"> Secondary Objective: Secondary Objective</span></h2>
<p>We have also been continuing the work of <a href="https://2007.igem.org/Carnegie_Mellon/Yoghurt" class="external text" title="https://2007.igem.org/Carnegie_Mellon/Yoghurt" rel="nofollow">our 2007 team</a> in engineering <i>Escherichia coli</i> to <b>produce the vitamin A precursor β-carotene</b>. Vitamin A is required for vision and a healthy immune system. 250,000-500,000 children in the developing world lose their vision each year, half of them dying within 12 months of this (<a href="http://www.who.int/nutrition/topics/vad/en/" class="external text" title="http://www.who.int/nutrition/topics/vad/en/" rel="nofollow">WHO</a>).
<p>We have also been continuing the work of <a href="https://2007.igem.org/Carnegie_Mellon/Yoghurt" class="external text" title="https://2007.igem.org/Carnegie_Mellon/Yoghurt" rel="nofollow">our 2007 team</a> in engineering <i>Escherichia coli</i> to <b>produce the vitamin A precursor β-carotene</b>. Vitamin A is required for vision and a healthy immune system. 250,000-500,000 children in the developing world lose their vision each year, half of them dying within 12 months of this (<a href="http://www.who.int/nutrition/topics/vad/en/" class="external text" title="http://www.who.int/nutrition/topics/vad/en/" rel="nofollow">WHO</a>).
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</p>

Revision as of 19:05, 22 May 2012

Team:Carnegie_Mellon - 20012.igem.org

Team:Carnegie_Mellon

From 2012.igem.org


Contents

Introduction: Motivation

  • We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure translational efficiency, and transcriptional strength.
  • We believe that we can use Spinach as a biosensor to reflect these metrics in vivo, rather than in vitro, which has previously proven to be very costly and impractical.
  • We will characterize the relationship between genetic expression of Spinach (upstream), translational efficiency, and transcriptional strength.


Place abstract/info here

Motivation question?

Humanistic implications go here

Primary Objective: A Useful BioBrick for Synthetic Biologists

A false-colour SEM of MicroMaize cells.
A false-colour SEM of MicroMaize cells.

We have been xxxxxyyyy for the purpose of xxxyyy Detailed by the following:

  1. something great
  2. something greater
  3. something awesome

This will be xxxyyy

Secondary Objective: Secondary Objective

We have also been continuing the work of our 2007 team in engineering Escherichia coli to produce the vitamin A precursor β-carotene. Vitamin A is required for vision and a healthy immune system. 250,000-500,000 children in the developing world lose their vision each year, half of them dying within 12 months of this (WHO).

Further Considerations

In the pursuit of our project, as well as the biological aspects, we: