Risk Assessments

In all cases correct laboratory practice should be observed and appropriate clothing and gloves worn. In some cases, additional precautions must be taken.

Colony PCR

Chemically competent cell - generation

Electrocompetent cell - generation

Electroporation

Gel electrophoresis

Gel extraction of DNA

Gibson Assembly

Glycerol Stocks

LB agar plates - preparation

Miniprep - DNA extraction

PCR using high temperature DNA polymerase

SDS PAGE protein analysis

Restriction Enzyme Digest

Transformation of Bacillus subtilis

Transformation of Escherichia coli

Use of Sodium Fluoride for construct testing