Colony PCR
Chemically competent cell - generation
Electrocompetent cell - generation
Electroporation
Gel electrophoresis
Gel extraction of DNA
Gibson Assembly
Glycerol Stocks
LB agar plates - preparation
Miniprep - DNA extraction
PCR using high temperature DNA polymerase
SDS PAGE protein analysis
Restriction Enzyme Digest
Transformation of Bacillus subtilis
Transformation of Escherichia coli
Use of Sodium Fluoride for construct testing