Team:Cambridge/Protocols/beta-galactosidaseassay

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Revision as of 17:33, 15 August 2012

β-galactosidase Assay

Theory

Protocol

  • Take suspension of cells grown up overnight and dilute 200 times in appropriate growth medium (for example LB broth for e.coli). If genetic construct can be selected for by an antibiotic, include this in the growth medium according to needs - this prevents loss of the plasmid during growth.
  • Now add the following to each of your eppindorf tubes:
' ReagentVolume (µl)
X-Gal (10mg/ml)200
Substance of interest (e.g. Fluoride) (0.5 M)1 for each mM desired in final solution
Cell suspensionMake up to 500 μl