"
Team:Cambridge/Protocols/SOB
From 2012.igem.org
(Difference between revisions)
Pdmallaband (Talk | contribs) (Created page with "{{Template:Team:Cambridge/CAM_2012_TEMPLATE_HEAD_DIARY}} A rich medium for cell growth =SOB= *0.5% (w/v) yeast extract *2% (w/v) tryptone *10 mM NaCl *2.5 mM KCl *20 mM MgSO<su...") |
Pdmallaband (Talk | contribs) |
||
| Line 19: | Line 19: | ||
*Some formulations of SOB use 10 mM MgCl<sub>2</sub> and 10 mM MgSO<sub>4</sub> instead of 20 mM MgSO<sub>4</sub>. | *Some formulations of SOB use 10 mM MgCl<sub>2</sub> and 10 mM MgSO<sub>4</sub> instead of 20 mM MgSO<sub>4</sub>. | ||
*Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter. | *Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter. | ||
| + | |||
| + | {{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}} | ||
Revision as of 18:23, 23 August 2012
A rich medium for cell growth
SOB
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
Per liter:
- 5 g yeast extract
- 20 g tryptone
- 0.584 g NaCl
- 0.186 g KCl
- 2.4 g MgSO4
- Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.
- Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.
