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Team:Cambridge/Protocols/SOB
From 2012.igem.org
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NOTE: Do not autoclave SOC as the glucose can react with the peptides - use filter sterilisation instead! | NOTE: Do not autoclave SOC as the glucose can react with the peptides - use filter sterilisation instead! | ||
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Revision as of 23:07, 29 August 2012
SOB
Super Optimal Broth - A rich medium for cell growth
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
Per liter:
- 5 g yeast extract
- 20 g tryptone
- 0.584 g NaCl
- 0.186 g KCl
- 2.4 g MgSO4
- Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.
- Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.
SOC
Super Optimal broth with Catabolite repression
If you need SOC, just add glucose to a final concentration of 10mM (3.603g in a litre). This is good recovery medium for the chemically competent cells transformation protocol.
NOTE: Do not autoclave SOC as the glucose can react with the peptides - use filter sterilisation instead!
NOTE 2: Store at -20°C
