Team:Cambridge/Protocols/SOB
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*Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter. | *Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter. | ||
- | ==SOC== | + | ===SOC=== |
If you need SOC, just add glucose to a final concentration of 10mM (3.603g in a litre). This is good recovery medium for the chemically competent cells transformation protocol. | If you need SOC, just add glucose to a final concentration of 10mM (3.603g in a litre). This is good recovery medium for the chemically competent cells transformation protocol. | ||
{{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}} | {{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}} |
Revision as of 09:17, 24 August 2012
SOB
A rich medium for cell growth
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
Per liter:
- 5 g yeast extract
- 20 g tryptone
- 0.584 g NaCl
- 0.186 g KCl
- 2.4 g MgSO4
- Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.
- Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.
SOC
If you need SOC, just add glucose to a final concentration of 10mM (3.603g in a litre). This is good recovery medium for the chemically competent cells transformation protocol.