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Team:Cambridge/Protocols/SOB
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A rich medium for cell growth | A rich medium for cell growth | ||
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*0.5% (w/v) yeast extract | *0.5% (w/v) yeast extract | ||
*2% (w/v) tryptone | *2% (w/v) tryptone | ||
Revision as of 18:23, 23 August 2012
SOB
A rich medium for cell growth
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
Per liter:
- 5 g yeast extract
- 20 g tryptone
- 0.584 g NaCl
- 0.186 g KCl
- 2.4 g MgSO4
- Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.
- Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.
