Team:Cambridge/Protocols/SOB
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*Some formulations of SOB use 10 mM MgCl<sub>2</sub> and 10 mM MgSO<sub>4</sub> instead of 20 mM MgSO<sub>4</sub>. | *Some formulations of SOB use 10 mM MgCl<sub>2</sub> and 10 mM MgSO<sub>4</sub> instead of 20 mM MgSO<sub>4</sub>. | ||
*Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter. | *Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter. | ||
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+ | {{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}} |
Revision as of 18:23, 23 August 2012
A rich medium for cell growth
SOB
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
Per liter:
- 5 g yeast extract
- 20 g tryptone
- 0.584 g NaCl
- 0.186 g KCl
- 2.4 g MgSO4
- Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.
- Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.