Team:Cambridge/Protocols/MediumB
From 2012.igem.org
Medium B
10X Medium A base:
- Yeast extract 10g
- Casamino acids 2g
- Distilled water to 900mL
- Autoclave then add:
- 50% glucose, filter sterilized 100mL
10X Bacillus salts:"
- (NH4)2SO4.3H2O 20g
- K2HPO4.3H2O 183g
- KH2PO4 60g
- Tri-sodium citrate 10g
- MgSO4.7H2O 2g
- SDW to 1000mL
Medium A
- Sterile water 81mL
- 10X Medium A base 10mL
- 10X Bacillus salts 9mL
- L-Tryptophan (11mg/mL) 0.1mL
Medium B
- Medium A 10mL
- 50mM CaCl2.2H2O 0.1mL
- 250nM MgCl2.6H2O 0.1mL
Important
- Autoclave Medium A base before adding glucose, and autoclave Bacillus salts.
- Store aliquots of 10X Medium A base 10mL and 10X Bacillus salts 9mL and keep them in the fridge, never use them twice to avoid contamination