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Team:Cambridge/Protocols/M9Medium
From 2012.igem.org
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*2mM MgSO<sub>4</sub> | *2mM MgSO<sub>4</sub> | ||
*0.1mM CaCl<sub>2</sub> | *0.1mM CaCl<sub>2</sub> | ||
| - | *0.4% carbon source (e.g. glycerol, glucose, etc.) | + | *0.4% carbon source (e.g. glycerol, glucose, etc.) - filter sterilized. |
*In sterile H<sub>2</sub>O | *In sterile H<sub>2</sub>O | ||
| + | |||
| + | '''NB - Do not autoclave final medium - carbon source will caramelize. | ||
{{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}} | {{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}} | ||
Revision as of 10:19, 18 September 2012
M9 Minimal Medium
M9 salts x5
- 64 g Na2HPO4.7H2O
- 15 g KH2PO4
- 2.5 g NaCl
- 5 g NH4Cl
dissolved in deionized water to a final volume of 1 L
Autoclave before use.
Final medium
- 1x M9 salts
- 2mM MgSO4
- 0.1mM CaCl2
- 0.4% carbon source (e.g. glycerol, glucose, etc.) - filter sterilized.
- In sterile H2O
NB - Do not autoclave final medium - carbon source will caramelize.
