Team:Cambridge/Protocols/M9Medium

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(Difference between revisions)
(Created page with "==M9 Minimal Medium== ''' M9 salts x5 ''' *64 g Na<sub>2</sub>HPO<sub>4</sub>.7H<sub>2</sub>O *15 g KH<sub>2</sub>PO<sub>4</sub> *2.5 g NaCl *5 g NH<sub>4</sub>Cl dissolved in...")
(M9 Minimal Medium)
Line 14: Line 14:
'''Final medium'''
'''Final medium'''
-
*1x [[M9 salts]]
+
*1x M9 salts
*2mM MgSO<sub>4</sub>
*2mM MgSO<sub>4</sub>
*0.1mM CaCl<sub>2</sub>
*0.1mM CaCl<sub>2</sub>
*0.4% carbon source (e.g. glycerol, glucose, etc.)
*0.4% carbon source (e.g. glycerol, glucose, etc.)
*In sterile H<sub>2</sub>O
*In sterile H<sub>2</sub>O

Revision as of 10:17, 18 September 2012

M9 Minimal Medium

M9 salts x5

  • 64 g Na2HPO4.7H2O
  • 15 g KH2PO4
  • 2.5 g NaCl
  • 5 g NH4Cl

dissolved in deionized water to a final volume of 1 L

Autoclave before use.

Final medium

  • 1x M9 salts
  • 2mM MgSO4
  • 0.1mM CaCl2
  • 0.4% carbon source (e.g. glycerol, glucose, etc.)
  • In sterile H2O