Producing high yields of B.subtilis spores

Preparation

Plate and grow B. subtilis overnight on antibiotic resistant plates.

Method

• Pick a single colony from a new plate of B. subtilis and use to inoculate 50ml CDSM.
• Grow this for 11 hours in a 37^oC incubator with vigorous aeration.
• Transfer 2-5% of this to make up another 50ml volume of CDSM culture and grow both for a total incubation time of 48 hours.
• Longer incubation may be needed for full release of spores.