Team:Cambridge/Protocols/GetSpores

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Producing high yields of B.subtilis spores

Preparation

Plate and grow B. subtilis overnight on antibiotic resistant plates.

Method

  • Pick a single colony from a new plate of B. subtilis and use to inoculate 50ml CDSM.
  • Grow this for 11 hours in a 37°C incubator with vigorous aeration.
  • Transfer 2.5mL of this to 70ml of new CDSM culture. Incubate new CDSM culture and original 50mL CDSM culture for a further 37hrs, to a total of 48hrs.
  • Longer incubation may be needed for full release of spores.