"
Team:Cambridge/Protocols/GetSpores
From 2012.igem.org
(Difference between revisions)
Pdmallaband (Talk | contribs) (→Method) |
(→Method) |
||
| Line 9: | Line 9: | ||
==Method== | ==Method== | ||
| - | *Pick a | + | *Pick a single colony from a new plate of B. subtilis and use to inoculate 50ml [[Team:Cambridge/Protocols/SporeMedia|<u><span style="color:#00000CD">CDSM</span></u>]]. |
*Grow this for 11 hours in a 37<sup>o</sup>C incubator with vigorous aeration. | *Grow this for 11 hours in a 37<sup>o</sup>C incubator with vigorous aeration. | ||
*Transfer 2-5% of this to make up another 50ml volume of [[Team:Cambridge/Protocols/SporeMedia|<u><span style="color:#00000CD">CDSM</span></u>]] culture and grow both for a total incubation time of 48 hours. | *Transfer 2-5% of this to make up another 50ml volume of [[Team:Cambridge/Protocols/SporeMedia|<u><span style="color:#00000CD">CDSM</span></u>]] culture and grow both for a total incubation time of 48 hours. | ||
Revision as of 12:05, 14 September 2012
Producing high yields of B.subtilis spores
Preparation
Plate and grow B. subtilis overnight on antibiotic resistant plates.
Method
- Pick a single colony from a new plate of B. subtilis and use to inoculate 50ml CDSM.
- Grow this for 11 hours in a 37oC incubator with vigorous aeration.
- Transfer 2-5% of this to make up another 50ml volume of CDSM culture and grow both for a total incubation time of 48 hours.
- Longer incubation may be needed for full release of spores.
