Team:Cambridge/Protocols/CCMB80

CCMB80 buffer

• 10 mM KOAc pH 7.0 (10 ml of a 1M stock/L or 0.98g/L)
• 80 mM CaCl₂.2H₂O (11.8 g/L)
• 20 mM MnCl₂.4H₂O (4.0 g/L)
• 10 mM MgCl₂.6H₂O (2.0 g/L or 5ml of 2M stock/L)
• 10% glycerol (100 ml/L)
• Fill up with DI water AFTER regulating pH

• adjust pH DOWN to 6.4 with 0.1N HCl if necessary
  • adjusting pH up will precipitate manganese dioxide from Mn containing solutions.
• sterile filter and store at 4°C
• slight dark precipitate appears not to affect its function