Team:Cambridge/Protocols

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Contents

Protocols

Construct production

Transformation protocols

Construct verification

Ribosense testing

  • β-galactosidase assay Assay to measure the amount of the enzyme β-galactosidase being produced by a population of cells. Useful as a reporter system.

Ratiometrica testing

  • IPTG induction A technique for inducing the pSPANK promoter in E.coli cells.

Miscellaneous

Recipes


Safety

See our Safety Page for Associated risk assessments for the above protocols and MSDS sheets for the reagents we have used.

Lab supplies


Other resources

File:Concentration calculator for lazy scientists.xls