Team:Cambridge/Project

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Contents

Overall project

Abstract

Previous iGEM teams have charaterised an impressive array of inducible promoters, along with other elements of biosensing circuitry. But, to date, the output from each is not consistent and, in spite of the unifying biobrick standards used, do not necessarily couple together to make integrated test kits. The Cambridge iGEM 2012 team aim to take the true meaning of biobricks to heart, by creating an open and applied biosensor standard available for use by all subsequent teams, as well as, potentially, by industry and researchers in the field.

The biosensor aims to be modular in design, allowing the kits to be tailored to an individual's requirements, and to use light as an output to allow computer interfacing. We aim to use two luciferases, one to give a read-out of the input, and the other to act as a standard to allow fluctuations in colony size to be taken into account. Furthermore, we shall be using B. subtilis as our chassis, with the view to making the most of the spore forming capacity of bacteria to send out desiccated kits with long shelf lives.

  • Update We have found a candidate for a new biobrick to be submitted to the registry of parts. Baker et al, in their paper entitled 'Widespread Genetic Switches and Toxicity Resistance Proteins for Fluoride', have identified a fluoride sensitive riboswitch which we, the Cambridge iGEM 2012 team, feel would be an excellent means to test, as proof of concept, our biosensor design.

Implementation

Project Details

Part 2

The Experiments

Part 3

Results