Team:Cambridge/Lab book/Week 8

From 2012.igem.org

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(Wednesday (15/08/12))
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===Wednesday (15/08/12)===
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'''[[Team:Cambridge/Protocols/PCRProtocol|PCR of positive control fragments]]'''
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----
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*
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*Standard PCR settings used
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*
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'''[[Team:Cambridge/Protocols/GelExtractionofDNA|Extraction of positive control DNA]]'''
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----
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*Positive control DNA from gel excised and purified.
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*Additional elution steps used to concentrate resultant solution.
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*Verified with nanodropper - final DNA concentrations:
===Thursday (16/08/12)===
===Thursday (16/08/12)===

Revision as of 10:06, 15 August 2012

Week: 3 4 5 6 7 8 9

Contents

Monday (13/08/12)

Tuesday (14/08/12)

Wednesday (15/08/12)

PCR of positive control fragments


  • Standard PCR settings used

Extraction of positive control DNA


  • Positive control DNA from gel excised and purified.
  • Additional elution steps used to concentrate resultant solution.
  • Verified with nanodropper - final DNA concentrations:

Thursday (16/08/12)

Friday (17/08/12)