Team:Cambridge/Lab book/Week 6

From 2012.igem.org

Revision as of 10:44, 30 July 2012 by Blipflip (Talk | contribs)

Week: 3 4 5 6 7

Contents

Monday

Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive.

PCR cycle x35 : *15s Denaturing at 95 C

               *45s Annealing at 60 C
               *300s Extension at 72 C

Tuesday

Wednesday

Thursday

Friday