Team:Cambridge/Lab book/Week 6

From 2012.igem.org

(Difference between revisions)
(Monday)
(Monday)
Line 16: Line 16:
PCR cycle x35:  
PCR cycle x35:  
-
15s Denaturing at 95 C
+
* 15s Denaturing at 95 C
-
45s Annealing at 60 C
+
* 45s Annealing at 60 C
-
300s Extension at 72 C
+
* 300s Extension at 72 C
 +
 
 +
The remaining stray product had a slightly tricky secondary structure at the 3' end. It will be run at a series of annealing temperatures in a PCR machine capable of a temperature gradient.
===Tuesday===
===Tuesday===

Revision as of 10:50, 30 July 2012

Week: 3 4 5 6 7

Contents

Monday

Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive.

PCR cycle x35:

  • 15s Denaturing at 95 C
  • 45s Annealing at 60 C
  • 300s Extension at 72 C

The remaining stray product had a slightly tricky secondary structure at the 3' end. It will be run at a series of annealing temperatures in a PCR machine capable of a temperature gradient.

Tuesday

Wednesday

Thursday

Friday