Team:Cambridge/Lab book/Week 6

From 2012.igem.org

(Difference between revisions)
(Monday)
(Monday)
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PCR cycle x35 : 15s Denaturing at 95 C
PCR cycle x35 : 15s Denaturing at 95 C
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                 45s Annealing
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                 45s Annealing at 60 C
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                 300s Extension
+
                 300s Extension at 72 C
===Tuesday===
===Tuesday===

Revision as of 10:43, 30 July 2012

Week: 3 4 5 6 7

Contents

Monday

Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive.

PCR cycle x35 : 15s Denaturing at 95 C

               45s Annealing at 60 C
               300s Extension at 72 C

Tuesday

Wednesday

Thursday

Friday