Team:Cambridge/Lab book/Week 11
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===Wednesday (05/09/12)=== | ===Wednesday (05/09/12)=== | ||
- | [[File: | + | [[File:BBPCR1.jpg|250px|left|thumb|PCR products from Wednesday's PCR. Lanes 2 - 3: Fluoride riboswitch biobrick genomic DNA. Lanes 4 - 5: MGRS construct genomic DNA (+8). Lanes 6 - 7: MGRS construct genomic DNA (-8). Lane 8: MGRS biobrick genomic DNA (+8).]] |
'''[[Team:Cambridge/Protocols/PCRProtocol|PCR of biobrick fragments and MGRS construct fragments.]]''' | '''[[Team:Cambridge/Protocols/PCRProtocol|PCR of biobrick fragments and MGRS construct fragments.]]''' | ||
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*Fluoride and magnesium riboswitch genomic DNA amplified with PCR, along with vector fragments for turning the MGRS into a biobrick and for producing the magnesium riboswitch construct. | *Fluoride and magnesium riboswitch genomic DNA amplified with PCR, along with vector fragments for turning the MGRS into a biobrick and for producing the magnesium riboswitch construct. | ||
*PCR settings: Annealing temperature - 58 °C, Elongation step - 60 seconds. | *PCR settings: Annealing temperature - 58 °C, Elongation step - 60 seconds. | ||
- | * | + | *Genomic fragments worked well, but longer backbone fragments mostly failed. |
===Thursday (06/09/12)=== | ===Thursday (06/09/12)=== |
Revision as of 15:02, 11 September 2012
Week: | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
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Contents |
Monday (03/09/12)
Tuesday (04/09/12)
Wednesday (05/09/12)
PCR of biobrick fragments and MGRS construct fragments.
- Fluoride and magnesium riboswitch genomic DNA amplified with PCR, along with vector fragments for turning the MGRS into a biobrick and for producing the magnesium riboswitch construct.
- PCR settings: Annealing temperature - 58 °C, Elongation step - 60 seconds.
- Genomic fragments worked well, but longer backbone fragments mostly failed.
Thursday (06/09/12)
Friday (07/09/12)
Saturday (08/09/12)
Gel for 9kb fragments of lux and flu construct:
File:Long frags PM 08.09.12.tif
Sunday (09/09/12)
Verification gel: