"
Team:Cambridge/Lab book/Week 11
From 2012.igem.org
(Difference between revisions)
(→Wednesday (05/09/12)) |
|||
| Line 20: | Line 20: | ||
===Wednesday (05/09/12)=== | ===Wednesday (05/09/12)=== | ||
| + | |||
| + | [[File:Example.jpg]] | ||
| + | |||
| + | '''[[Team:Cambridge/Protocols/PCRProtocol|PCR of biobrick fragments and MGRS construct fragments.]]''' | ||
| + | |||
| + | ---- | ||
| + | |||
| + | *Fluoride and magnesium riboswitch genomic DNA amplified with PCR, along with vector fragments for turning the MGRS into a biobrick and for producing the magnesium riboswitch construct. | ||
| + | *PCR settings: Annealing temperature - 58 °C, Elongation step - 60 seconds. | ||
| + | * | ||
===Thursday (06/09/12)=== | ===Thursday (06/09/12)=== | ||
Revision as of 14:56, 11 September 2012
| Week: | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
|---|
Contents |
Monday (03/09/12)
Tuesday (04/09/12)
Wednesday (05/09/12)
PCR of biobrick fragments and MGRS construct fragments.
- Fluoride and magnesium riboswitch genomic DNA amplified with PCR, along with vector fragments for turning the MGRS into a biobrick and for producing the magnesium riboswitch construct.
- PCR settings: Annealing temperature - 58 °C, Elongation step - 60 seconds.
Thursday (06/09/12)
Friday (07/09/12)
Saturday (08/09/12)
Gel for 9kb fragments of lux and flu construct:
File:Long frags PM 08.09.12.tif
Sunday (09/09/12)
Verification gel:
