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Team:Cambridge/Diary/Week 7
From 2012.igem.org
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Contents |
Monday
Like many days, today was a good day for shopping.
Oli re-ran his vector fragment B PCR and, despite some slightly weak bands, extracted the DNA needed.
We've run out of competent e.coli cells, and we need some more. Unfortunately, the TOP10 cells that were grown up over the weekend were too old to use, so Paul is using our final vial of TOP10 to make some more that should be in the log phase by tomorrow morning. Then we can get on with the protocol to make them competent.
Tuesday
A very practical day today, as various people (Paul and Andreas in particular) undertook to perform the rather long electro-competent cell production protocol. We also recieved our delivery of reagents we ordered yesterday. Using some rather stinky NAD+, the isothermal reaction buffer for the Gibson assembly reaction was finished off and lots of aliquots of Gibson master mix stored.
Wednesday
Thursday
Friday
