Team:Cambridge/Diary/Week 7

From 2012.igem.org

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===Tuesday===
===Tuesday===
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A very practical day today, as various people (Paul and Andreas in particular) undertook to perform the rather long [[Team:Cambridge/Protocols/Electrocompetentcells|electro-competent cell production protocol]]. We also recieved our delivery of reagents we ordered yesterday. Using some rather stinky NAD+, the isothermal reaction buffer for the Gibson assembly reaction was finished off and lots of aliquots of Gibson master mix stored.
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A very practical day today, as various people (Paul and Andreas in particular) undertook to perform the rather long [[Team:Cambridge/Protocols/Electrocompetentcells|electro-competent cell production protocol]]. We also recieved our delivery of reagents we ordered yesterday. Using some quite stinky NAD+, the isothermal reaction buffer for the Gibson assembly reaction was finished off and lots of aliquots of Gibson master mix stored.
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Having made all this, we did some more Gibson, which *should* work this time. Certainly, we're pretty confident we have all the ingredients in the mix this time. We'll transform some cells with the resultant DNA cocktail as soon as we have some competent cells. Hopefully tomorrow then, though such efficient hopes have had a way of failing in the last couple of weeks.
===Wednesday===
===Wednesday===

Revision as of 09:56, 8 August 2012

Week: 1 2 3 4 5 6 7

Contents

Monday

Like many days, today was a good day for shopping.

Oli re-ran his vector fragment B PCR and, despite some slightly weak bands, extracted the DNA needed.

We've run out of competent e.coli cells, and we need some more. Unfortunately, the TOP10 cells that were grown up over the weekend were too old to use, so Paul is using our final vial of TOP10 to make some more that should be in the log phase by tomorrow morning. Then we can get on with the protocol to make them competent.

Tuesday

A very practical day today, as various people (Paul and Andreas in particular) undertook to perform the rather long electro-competent cell production protocol. We also recieved our delivery of reagents we ordered yesterday. Using some quite stinky NAD+, the isothermal reaction buffer for the Gibson assembly reaction was finished off and lots of aliquots of Gibson master mix stored.

Having made all this, we did some more Gibson, which *should* work this time. Certainly, we're pretty confident we have all the ingredients in the mix this time. We'll transform some cells with the resultant DNA cocktail as soon as we have some competent cells. Hopefully tomorrow then, though such efficient hopes have had a way of failing in the last couple of weeks.

Wednesday

Thursday

Friday