Team:Cambridge/Diary/Week 13
From 2012.igem.org
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===Tuesday=== | ===Tuesday=== | ||
*Ran PCR for making SPOBB biobrick, gel yielded insufficient results. Redesigned and reordered primers | *Ran PCR for making SPOBB biobrick, gel yielded insufficient results. Redesigned and reordered primers | ||
+ | *Ratiometrica: rough IPTG induction with 8 different concentrations of IPTG, after 3 hours of incubation there was unfortunately no obvious results (i.e. no visible difference between different concentrations/induced and uninduced smaples), will leave them growing overnight and observe again tomorrow; sequencing primers arrived so the plasmid is sent off to sequencing, will have results tomorrow morning; more O/N cultures grown for miniprep to put into Bacillus tomorrow. | ||
===Wednesday=== | ===Wednesday=== |
Revision as of 07:52, 19 September 2012
Week: | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 |
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Contents |
Monday
- Ratiometrica: grow O/N cultures for rough IPTG induction tomorrow, ordered primers to convert into biobrick
Tuesday
- Ran PCR for making SPOBB biobrick, gel yielded insufficient results. Redesigned and reordered primers
- Ratiometrica: rough IPTG induction with 8 different concentrations of IPTG, after 3 hours of incubation there was unfortunately no obvious results (i.e. no visible difference between different concentrations/induced and uninduced smaples), will leave them growing overnight and observe again tomorrow; sequencing primers arrived so the plasmid is sent off to sequencing, will have results tomorrow morning; more O/N cultures grown for miniprep to put into Bacillus tomorrow.
Wednesday
Thursday
Friday
Saturday
Sunday