Team:Cambridge/Diary/Week 12

From 2012.igem.org

Revision as of 12:26, 14 September 2012 by Olijme (Talk | contribs)

Week: 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Contents

Monday

PCR of the biobrick backbone is still proving troublesome, as no bands are coming out. Oli's trying the PCRs at ever higher

But at least both a restriction digest and a colony PCR confirmed the identity of our Magnesium Riboswitch construct. One of the colonies seems to be fluorescing far more green than the other, which may be down to a mutation that has upregulated our sfGFP. We'll need to do some characterization before we can confirm that, though.

Tuesday

Wednesday

Thursday

Friday

Analysis of the sequence sent to us of the biobricks we had hoped to make indicate that the riboswitches have inserted - backwards. The current sequence is Backbone-Front of Suffix-End of Prefix-Riboswitch-End of Suffix-Start of Prefix. This almost success is somewhat mocking. However, Jolyon is starting to try to assemble our biobricks with standard assembly instead - hopefully less can go wrong with this. It's a shame to give up on Gibson, but it has not been our friend this Summer.