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Team:Cambridge/Diary/Week 12
From 2012.igem.org
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Contents |
Monday
PCR of the biobrick backbone is still proving troublesome, as no bands are coming out. Oli's trying the PCRs at ever higher
But at least both a restriction digest and a colony PCR confirmed the identity of our Magnesium Riboswitch construct. One of the colonies seems to be fluorescing far more green than the other, which may be down to a mutation that has upregulated our sfGFP. We'll need to do some characterization before we can confirm that, though.
Tuesday
Wednesday
Thursday
Friday
