Team:Cambridge

From 2012.igem.org

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<p><h9>THE DRIVING FORCE</h9>
<p><h9>THE DRIVING FORCE</h9>
<p><h9>Our goal is to standardise the output of biosensors, and to develop a cheap, user-friendly kit to be used in the field</h9>
<p><h9>Our goal is to standardise the output of biosensors, and to develop a cheap, user-friendly kit to be used in the field</h9>
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<p><h9> >>Overview</h9>
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<p><a href="/Team:Cambridge/HumanPractices/Overview"><h9> >>Overview</h9></a>
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<p><h9> >>Market Research</h9>
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<p><a href="/Team:Cambridge/HumanPractices/MarketResearch"><h9> >>Market Research</h9>
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<p><h9> >>Outreach & Collaborations</h9>
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<p><a href="/Team:Cambridge/HumanPractices/OutreachCollaboration"><h9> >>Outreach & Collaborations</h9>
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<p><h9> >>Future Directions </h9>
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<p><a href="/Team:Cambridge/HumanPractices/FutureDirections"><h9> >>Future Directions </h9>
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<p><h9>There are many biosensors available but there is no standard way to deploy them. Many are also non-quantitative and unpredictable. We have been working on a ratiometric luciferase output which can be read by an Arduino device.
<p><h9>There are many biosensors available but there is no standard way to deploy them. Many are also non-quantitative and unpredictable. We have been working on a ratiometric luciferase output which can be read by an Arduino device.
This output could be used with theoretically any biosensor. We investigated the potential of riboswitches as future biosensors. We use a B.subtilis chassis as these form low maintenance spores for easy, long-term storage. </h9>
This output could be used with theoretically any biosensor. We investigated the potential of riboswitches as future biosensors. We use a B.subtilis chassis as these form low maintenance spores for easy, long-term storage. </h9>
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<p><h9> >>Overview</h9>
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<p><a href="/Team:Cambridge/Overview/Overview"><h9> >>Overview</h9></a>
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<p><h9> >>Design Process</h9>
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<p><a href="/Team:Cambridge/Overview/DesignProcess"><h9> >>Design Process</h9></a>
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<p><h9> >>Parts</h9>
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<p><a href="/Team:Cambridge/Overview/Parts"><h9> >>Parts</h9></a>
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<p><h9> >>Lab book</h9>
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<p><a href="/Team:Cambridge/Overview/Labbook"><h9> >>Lab book</h9></a>
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<p><h9> >>Results</h9>
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<p><a href="/Team:Cambridge/Overview/Results"><h9> >>Results</h9></a>
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<p><h9>INVESTIGATING THE POTENTIAL OF RATIOMETRIC REPORTER CONSTRUCTS FOR ACCURATE AND REPRODUCIBLE CHARACTERISATION</h9>
<p><h9>INVESTIGATING THE POTENTIAL OF RATIOMETRIC REPORTER CONSTRUCTS FOR ACCURATE AND REPRODUCIBLE CHARACTERISATION</h9>
<p><h9>We designed fluorescence and luminescence-based constructs that would function in both E.coli and B.subtilis</h9>
<p><h9>We designed fluorescence and luminescence-based constructs that would function in both E.coli and B.subtilis</h9>
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<p><h9> >>Overview</h9>
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<p><a href="/Team:Cambridge/Ratiometrica/Overview"><h9> >>Overview</h9>
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<p><h9> >>Design Process</h9>
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<p><a href="/Team:Cambridge/Ratiometrica/DesignProcess"><h9> >>Design Process</h9>
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<p><h9> >>Parts</h9>
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<p><a href="/Team:Cambridge/Ratiometrica/Parts"><h9> >>Parts</h9>
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<p><h9> >>Lab book</h9>
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<p><a href="/Team:Cambridge/Ratiometrica/Labbook"><h9> >>Lab book</h9>
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<p><h9> >>Results</h9>
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<p><a href="/Team:Cambridge/Ratiometrica/Results"><h9> >>Results</h9>
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Revision as of 22:16, 26 October 2012

Human Practices

THE DRIVING FORCE

Our goal is to standardise the output of biosensors, and to develop a cheap, user-friendly kit to be used in the field

>>Overview

>>Market Research

>>Outreach & Collaborations

>>Future Directions

RiboSense

A NOVEL FLUORIDE SENSOR BASED ON A RIBOSWITCH CONSTRUCT

Riboswitches may well be the biosensor of the future though they are currently under-represented in the registry.

>>Overview

>>Design Process

>>Parts

>>Lab book

>>Results

Ratiometrica

INVESTIGATING THE POTENTIAL OF RATIOMETRIC REPORTER CONSTRUCTS FOR ACCURATE AND REPRODUCIBLE CHARACTERISATION

We designed fluorescence and luminescence-based constructs that would function in both E.coli and B.subtilis

>>Overview

>>Design Process

>>Parts

>>Lab book

>>Results

Sporage & Distribution

B. SUBTILIS: LONG TERM, USER-FRIENDLY STORAGE

We developed and optimised procedures for sporulation and germination.

>>Overview

>>Design Process

>>Parts

>>Lab book

>>Results