http://2012.igem.org/wiki/index.php?title=Team:Caltech/Notebook/Safety&feed=atom&action=historyTeam:Caltech/Notebook/Safety - Revision history2024-03-29T05:28:42ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Caltech/Notebook/Safety&diff=262471&oldid=prevKknister at 00:25, 4 October 20122012-10-04T00:25:24Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h1> Safety Page </<del class="diffchange diffchange-inline">hy</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h1> Safety Page </<ins class="diffchange diffchange-inline">h1</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety Regulations==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety Regulations==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>International: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>International: </div></td></tr>
</table>Kknisterhttp://2012.igem.org/wiki/index.php?title=Team:Caltech/Notebook/Safety&diff=262466&oldid=prevKknister at 00:25, 4 October 20122012-10-04T00:25:09Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety Regulations==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety Regulations==</div></td></tr>
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</table>Kknisterhttp://2012.igem.org/wiki/index.php?title=Team:Caltech/Notebook/Safety&diff=102282&oldid=prevDlin at 06:52, 8 September 20122012-09-08T06:52:23Z<p></p>
<a href="http://2012.igem.org/wiki/index.php?title=Team:Caltech/Notebook/Safety&diff=102282&oldid=102149">Show changes</a>Dlinhttp://2012.igem.org/wiki/index.php?title=Team:Caltech/Notebook/Safety&diff=102149&oldid=prevCqiu at 05:05, 8 September 20122012-09-08T05:05:49Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Our new BioBricks parts consist of genes </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Our new BioBricks parts consist of genes </p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==New BioBricks==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Our new biobricks are Ampicillin and Chloramphenycol resistant and allow the creation of a proton gradient using only light. They are not hazardous in any way and quite useless in a wild type E. coli. The biobricks are well documented for submission into the Registry.</p></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>&nbsp;</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>&nbsp;</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><strong>Is there a local biosafety group, committee, or review board at your institution?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><strong>Is there a local biosafety group, committee, or review board at your institution?</div></td></tr>
</table>Cqiuhttp://2012.igem.org/wiki/index.php?title=Team:Caltech/Notebook/Safety&diff=102147&oldid=prevCqiu at 04:58, 8 September 20122012-09-08T04:58:40Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>We are transforming genes into non-pathogenic Escherichia coli and Zymomonas mobilis, which are biological safety level 1 organisms, using plasmids with various antibiotic resistances. Furthermore, we are attempting to isolate bacteria from the environment capable of degrading lignin, alginate, and polystyrene. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>We are transforming genes into non-pathogenic Escherichia coli and Zymomonas mobilis, which are biological safety level 1 organisms, using plasmids with various antibiotic resistances. Furthermore, we are attempting to isolate bacteria from the environment capable of degrading lignin, alginate, and polystyrene. </p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Researcher Safety==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>While most of our project is in lab strains of E. coli (NIH Risk Group 1), we are culturing organisms from environmental samples that are able to survive in minimal media containing one of compounds lignin, alginate, or polystyrene we are hoping to degrade. Due to immediate culturing in minimal media, it is unlikely that we will culture any significantly pathogenic organisms from our turtle pond on campus, according to the Caltech Safety Office.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>We are then transforming the genes capable of degrading those compounds into E. coli, using standard laboratory procedures. The strains are also non-pathogenic.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Once the bacteria degrades the compound, we then use bacteria that can eat the degraded polymers. These E. coli ferment and create ethanol, a form of bio-fuel. The strains we are using are not dangerous, but ethanol in high concentrations can pose a potential health hazard. Nevertheless, we are not producing ethanol in high enough concentration for major concerns.</P></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Public and Environmental Safety==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Because we isolated our organisms from a healthy environment, there is not much to worry about in terms of public safety. Our strains of E. coli are also typical laboratory strains, the bacteria is unlikely to survive in the environment. Nevertheless, we make sure to follow the bio-safety regulations for the engineered microbes, such as bleaching the cultures before disposing them. Our strains are not meant to be released into the wild.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>In the case that some cells do escape into the environment, a method of containing genetically modified bacteria is to prevent the recombinant plasmids from being taken up by native species (horizontal gene transfer) and to prevent the engineered strain from outcompeting the local community. A common method is to use the ccdB gene ([http://partsregistry.org/Part:BBa_P1016 BBa_P1016] and [http://partsregistry.org/Part:BBa_P1010 BBa_P1010])on a plasmid in conjunction with an E. coli strain such as DB3.1 ([http://partsregistry.org/Part:BBa_V1005 BBa_V1005]). The plasmid will code for the “death gene” which will kill any cell that does not code for immunity in its genome. If a native microorganism would uptake this man-made plasmid, it would die, preventing the propagation of the recombinant DNA in the environment (Featured Parts: Cell Death).</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Another similar “suicide” containment system uses streptavidin ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J36841 BBa_J36841]) (Kaplan, Mello et al. 1999; Urgun-Demirtas, Stark et al. 2006). This protein binds very tightly to biotin, a required co-enzyme for many metabolic pathways. This makes biotin unavailable and causes cell death. Kaplan et al. reported cell counts were reduced 99.9% in eight hours after their system was activated by absence of pollutant to degrade.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>None of the above is necessary because normal lab strains are engineered so that they will be unable to survive in the field.</p></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><strong> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><strong> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,</div></td></tr>
</table>Cqiuhttp://2012.igem.org/wiki/index.php?title=Team:Caltech/Notebook/Safety&diff=102116&oldid=prevCqiu at 04:34, 8 September 20122012-09-08T04:34:17Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Yes, Caltech has an Environment, Health, and Safety (EHS) Office. Our team completed safety training, including a walkthrough of the lab with the lab safety officer before beginning any lab work. We also spent about 3 days with our graduate student mentors learning molecular biology techniques and the safety measures associated with them. We talked to the Caltech safety office about our project. They saw no problems with our handling of biological safety since we are working with level 1 organisms. They noted that the possibility of growing pathological bacteria from our enrichment cultures is low due to immediately diluting in minimal media rather than growing environmental samples up on LB. If we suspect any of the cultured organisms are harmful, we will work with the safety office to implement proper safety procedures. Furthermore, we talked to the safety office about possible chemical hazards in our lab. Any solid waste (tubes, etc) should be put in a container or bag and also labeled as hazardous waste.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Yes, Caltech has an Environment, Health, and Safety (EHS) Office. Our team completed safety training, including a walkthrough of the lab with the lab safety officer before beginning any lab work. We also spent about 3 days with our graduate student mentors learning molecular biology techniques and the safety measures associated with them. We talked to the Caltech safety office about our project. They saw no problems with our handling of biological safety since we are working with level 1 organisms. They noted that the possibility of growing pathological bacteria from our enrichment cultures is low due to immediately diluting in minimal media rather than growing environmental samples up on LB. If we suspect any of the cultured organisms are harmful, we will work with the safety office to implement proper safety procedures. Furthermore, we talked to the safety office about possible chemical hazards in our lab. Any solid waste (tubes, etc) should be put in a container or bag and also labeled as hazardous waste.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Safety Regulations</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Safety Regulations</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>International: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>International: </div></td></tr>
</table>Cqiuhttp://2012.igem.org/wiki/index.php?title=Team:Caltech/Notebook/Safety&diff=102115&oldid=prevCqiu: /* Safety Regulations */2012-09-08T04:33:53Z<p><span class="autocomment">Safety Regulations</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Yes, Caltech has an Environment, Health, and Safety (EHS) Office. Our team completed safety training, including a walkthrough of the lab with the lab safety officer before beginning any lab work. We also spent about 3 days with our graduate student mentors learning molecular biology techniques and the safety measures associated with them. We talked to the Caltech safety office about our project. They saw no problems with our handling of biological safety since we are working with level 1 organisms. They noted that the possibility of growing pathological bacteria from our enrichment cultures is low due to immediately diluting in minimal media rather than growing environmental samples up on LB. If we suspect any of the cultured organisms are harmful, we will work with the safety office to implement proper safety procedures. Furthermore, we talked to the safety office about possible chemical hazards in our lab. Any solid waste (tubes, etc) should be put in a container or bag and also labeled as hazardous waste.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Yes, Caltech has an Environment, Health, and Safety (EHS) Office. Our team completed safety training, including a walkthrough of the lab with the lab safety officer before beginning any lab work. We also spent about 3 days with our graduate student mentors learning molecular biology techniques and the safety measures associated with them. We talked to the Caltech safety office about our project. They saw no problems with our handling of biological safety since we are working with level 1 organisms. They noted that the possibility of growing pathological bacteria from our enrichment cultures is low due to immediately diluting in minimal media rather than growing environmental samples up on LB. If we suspect any of the cultured organisms are harmful, we will work with the safety office to implement proper safety procedures. Furthermore, we talked to the safety office about possible chemical hazards in our lab. Any solid waste (tubes, etc) should be put in a container or bag and also labeled as hazardous waste.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://www.who.int/csr/delibepidemics/WHO_CDS_CSR_LYO_2004_11/en/ WHO] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://www.who.int/csr/delibepidemics/WHO_CDS_CSR_LYO_2004_11/en/ WHO] </div></td></tr>
</table>Cqiuhttp://2012.igem.org/wiki/index.php?title=Team:Caltech/Notebook/Safety&diff=102113&oldid=prevCqiu: /* Safety Regulations */2012-09-08T04:33:18Z<p><span class="autocomment">Safety Regulations</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Yes, Caltech has an Environment, Health, and Safety (EHS) Office. Our team completed safety training, including a walkthrough of the lab with the lab safety officer before beginning any lab work. We also spent about 3 days with our graduate student mentors learning molecular biology techniques and the safety measures associated with them. We talked to the Caltech safety office about our project. They saw no problems with our handling of biological safety since we are working with level 1 organisms. They noted that the possibility of growing pathological bacteria from our enrichment cultures is low due to immediately diluting in minimal media rather than growing environmental samples up on LB. If we suspect any of the cultured organisms are harmful, we will work with the safety office to implement proper safety procedures. Furthermore, we talked to the safety office about possible chemical hazards in our lab. Any solid waste (tubes, etc) should be put in a container or bag and also labeled as hazardous waste.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Yes, Caltech has an Environment, Health, and Safety (EHS) Office. Our team completed safety training, including a walkthrough of the lab with the lab safety officer before beginning any lab work. We also spent about 3 days with our graduate student mentors learning molecular biology techniques and the safety measures associated with them. We talked to the Caltech safety office about our project. They saw no problems with our handling of biological safety since we are working with level 1 organisms. They noted that the possibility of growing pathological bacteria from our enrichment cultures is low due to immediately diluting in minimal media rather than growing environmental samples up on LB. If we suspect any of the cultured organisms are harmful, we will work with the safety office to implement proper safety procedures. Furthermore, we talked to the safety office about possible chemical hazards in our lab. Any solid waste (tubes, etc) should be put in a container or bag and also labeled as hazardous waste.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">/</del>p>Safety Regulations<<del class="diffchange diffchange-inline">/</del>p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Safety Regulations<p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>International: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>International: </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://www.who.int/csr/delibepidemics/WHO_CDS_CSR_LYO_2004_11/en/ WHO] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://www.who.int/csr/delibepidemics/WHO_CDS_CSR_LYO_2004_11/en/ WHO] </div></td></tr>
</table>Cqiuhttp://2012.igem.org/wiki/index.php?title=Team:Caltech/Notebook/Safety&diff=102112&oldid=prevCqiu: /* Safety Regulations */2012-09-08T04:32:37Z<p><span class="autocomment">Safety Regulations</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Yes, Caltech has an Environment, Health, and Safety (EHS) Office. Our team completed safety training, including a walkthrough of the lab with the lab safety officer before beginning any lab work. We also spent about 3 days with our graduate student mentors learning molecular biology techniques and the safety measures associated with them. We talked to the Caltech safety office about our project. They saw no problems with our handling of biological safety since we are working with level 1 organisms. They noted that the possibility of growing pathological bacteria from our enrichment cultures is low due to immediately diluting in minimal media rather than growing environmental samples up on LB. If we suspect any of the cultured organisms are harmful, we will work with the safety office to implement proper safety procedures. Furthermore, we talked to the safety office about possible chemical hazards in our lab. Any solid waste (tubes, etc) should be put in a container or bag and also labeled as hazardous waste.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Yes, Caltech has an Environment, Health, and Safety (EHS) Office. Our team completed safety training, including a walkthrough of the lab with the lab safety officer before beginning any lab work. We also spent about 3 days with our graduate student mentors learning molecular biology techniques and the safety measures associated with them. We talked to the Caltech safety office about our project. They saw no problems with our handling of biological safety since we are working with level 1 organisms. They noted that the possibility of growing pathological bacteria from our enrichment cultures is low due to immediately diluting in minimal media rather than growing environmental samples up on LB. If we suspect any of the cultured organisms are harmful, we will work with the safety office to implement proper safety procedures. Furthermore, we talked to the safety office about possible chemical hazards in our lab. Any solid waste (tubes, etc) should be put in a container or bag and also labeled as hazardous waste.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></<ins class="diffchange diffchange-inline">p</ins>>Safety Regulations</<ins class="diffchange diffchange-inline">p</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div></<del class="diffchange diffchange-inline">h2</del>>Safety Regulations</<del class="diffchange diffchange-inline">h2</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>International: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>International: </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://www.who.int/csr/delibepidemics/WHO_CDS_CSR_LYO_2004_11/en/ WHO] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://www.who.int/csr/delibepidemics/WHO_CDS_CSR_LYO_2004_11/en/ WHO] </div></td></tr>
</table>Cqiuhttp://2012.igem.org/wiki/index.php?title=Team:Caltech/Notebook/Safety&diff=102107&oldid=prevCqiu: /* Safety Regulations */2012-09-08T04:31:10Z<p><span class="autocomment">Safety Regulations</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Yes, Caltech has an Environment, Health, and Safety (EHS) Office. Our team completed safety training, including a walkthrough of the lab with the lab safety officer before beginning any lab work. We also spent about 3 days with our graduate student mentors learning molecular biology techniques and the safety measures associated with them. We talked to the Caltech safety office about our project. They saw no problems with our handling of biological safety since we are working with level 1 organisms. They noted that the possibility of growing pathological bacteria from our enrichment cultures is low due to immediately diluting in minimal media rather than growing environmental samples up on LB. If we suspect any of the cultured organisms are harmful, we will work with the safety office to implement proper safety procedures. Furthermore, we talked to the safety office about possible chemical hazards in our lab. Any solid waste (tubes, etc) should be put in a container or bag and also labeled as hazardous waste.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Yes, Caltech has an Environment, Health, and Safety (EHS) Office. Our team completed safety training, including a walkthrough of the lab with the lab safety officer before beginning any lab work. We also spent about 3 days with our graduate student mentors learning molecular biology techniques and the safety measures associated with them. We talked to the Caltech safety office about our project. They saw no problems with our handling of biological safety since we are working with level 1 organisms. They noted that the possibility of growing pathological bacteria from our enrichment cultures is low due to immediately diluting in minimal media rather than growing environmental samples up on LB. If we suspect any of the cultured organisms are harmful, we will work with the safety office to implement proper safety procedures. Furthermore, we talked to the safety office about possible chemical hazards in our lab. Any solid waste (tubes, etc) should be put in a container or bag and also labeled as hazardous waste.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==</del>Safety Regulations<del class="diffchange diffchange-inline">==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></h2></ins>Safety Regulations<ins class="diffchange diffchange-inline"></h2></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>International: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>International: </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://www.who.int/csr/delibepidemics/WHO_CDS_CSR_LYO_2004_11/en/ WHO] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://www.who.int/csr/delibepidemics/WHO_CDS_CSR_LYO_2004_11/en/ WHO] </div></td></tr>
</table>Cqiu