Team:Calgary/Project/Post-Regionals

From 2012.igem.org

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<li><p><a class="orange" href="http://2012.igem.org/Team:Calgary/Project/HumanPractices/Interviews"><b>Returned to industry experts</b></a> to see if we accomplished our goals and what the <a class="purple" href="http://2012.igem.org/Team:Calgary/Project/Synergy"><b>next steps</b></a> should be.</li></p>
<li><p><a class="orange" href="http://2012.igem.org/Team:Calgary/Project/HumanPractices/Interviews"><b>Returned to industry experts</b></a> to see if we accomplished our goals and what the <a class="purple" href="http://2012.igem.org/Team:Calgary/Project/Synergy"><b>next steps</b></a> should be.</li></p>
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<li><p><a class="orange" href="http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>Characterized</b></a> the functionality of our previously submitted <a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902066"><b><i>Prha</i> (BBa_K902066)</b></a> promoter</b> using GFP fluorescence.</li></p>
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<li><p><a class="orange" href="http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>Characterized</b></a> the functionality of our previously submitted <a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902066"><b><i>Prha</i> (BBa_K902066)</b></a> promoter using GFP fluorescence.</li></p>
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<li><p>Tested an additional <b>complete kill switch device</b> using the <i>Prha</i> promoter with our S7 kill gene.</li></p>
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<li><p>Tested an additional <a class="orange" href="http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>complete kill switch device</b></a> using the <a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902084"><b><i>Prha</i> (BBa_K902084)</b></a> promoter with our S7 kill gene.</li></p>
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<li><p><b>Further characterized</b> our previously validated <b>magnesium riboswitch kill gene construct</b>.</li></p></ul>
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<li><p><a class="orange" href="http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>Further characterized</b></a> our previously validated <b>magnesium riboswitch kill gene construct</b><a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902018"> <b>(BBa_K902018)</b></a>.</li></p></ul>
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<li><p><b>Electrochemically characterized</b> an inducible <b><i>lacz</i> circuit</b>, replacing a faulty existing circuit in the registry containing a frameshift.</p></li>
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<li><p><a class="green" href="http://2012.igem.org/Team:Calgary/Project/FRED/Reporting"><b>Electrochemically characterized</b></a> an inducible <a class="green" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902090"><b><i>lacz</i> (BBa_K902090)</b></a> circuit, replacing a faulty existing circuit in the registry containing a frameshift.</p></li>
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<li><p><b>Electrochemically characterized</b> a constitutive <b>bglX generator</b> in terms of its electrochemical activity.</p></li>
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<li><p><a class="green" href="http://2012.igem.org/Team:Calgary/Project/FRED/Reporting"><b>Electrochemically characterized</b></a> a constitutive <a class="green" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902005"><b><i>bglX</i> (BBa_K902005)</b></a> <b>generator</b> in terms of its electrochemical activity.</p></li>
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<li><p><b>Characterized</b> and demonstrated how <b>3 different hydrolase-producing circuits</b> can be used simultaneously in order to create a <b>multiplex biosensor.</b> </p></li>
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<li><p><a class="green" href="http://2012.igem.org/Team:Calgary/Project/FRED/Reporting"><b>Characterized</b></a> and demonstrated how <b>3 different hydrolase-producing circuits</b> can be used simultaneously in order to create a <b>multiplex biosensor.</b> </p></li>
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<li><p><b>Tested one of our transposon library hits</b> with our electrochemical reporter, showing that we can use our system to <b>detect toxins electrochemically</b>.</p></li>
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<li><p><a class="purple" href="http://2012.igem.org/Team:Calgary/Project/Synergy"><b>Tested one of our transposon library hits</b></a> with our electrochemical reporter, showing that we can use our system to <b>detect toxins electrochemically</b>.</p></li>
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<p><b>In terms of <FONT COLOR=#1088CC>OCSAR</FONT>, we...</b></p>
<p><b>In terms of <FONT COLOR=#1088CC>OCSAR</FONT>, we...</b></p>
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<li><p>Obtained <b>additional characterization data</b> in validation of our flux-variability analysis model.</p></li>
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<li><p>Obtained <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/FluxAnalysis"><b>additional characterization data</b></a> in validation of our flux-variability analysis model.</p></li>
<li><p>Characterized the ability of our <b>novel amidase BioBrick</b> to remove nitrogen atoms from ring structures with remarkeable efficiency, turning them into a substrate that the Petrobrick (BBa_K) can likely convert into hydrocarbons.</p></li>
<li><p>Characterized the ability of our <b>novel amidase BioBrick</b> to remove nitrogen atoms from ring structures with remarkeable efficiency, turning them into a substrate that the Petrobrick (BBa_K) can likely convert into hydrocarbons.</p></li>

Revision as of 20:22, 26 October 2012

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Post-Regional Accomplishments

Our team has had many accomplishments since the regional jamboree!

In our Human Practices project, we...


In terms of FRED, we...


In terms of OCSAR, we...

  • Obtained additional characterization data in validation of our flux-variability analysis model.

  • Characterized the ability of our novel amidase BioBrick to remove nitrogen atoms from ring structures with remarkeable efficiency, turning them into a substrate that the Petrobrick (BBa_K) can likely convert into hydrocarbons.

  • Tested out our bioreactor and it’s ability to grow cells containing the PetroBrick as well as extract the hydrocarbon layer produced using our belt skimmer