Team:Calgary/Project/Post-Regionals

From 2012.igem.org

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<li><p><b>Returned to industry experts</b> to see if we accomplished our goals and what the <b>next steps</b> should be.</li></p>
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<li><p><a class="orange" href="http://2012.igem.org/Team:Calgary/Project/HumanPractices/Interviews"><b>Returned to industry experts</b></a> to see if we accomplished our goals and what the <a class="purple" href="http://2012.igem.org/Team:Calgary/Project/Synergy"><b>next steps</b></a> should be.</li></p>
<li><p><b>Characterized</b> the functionality of our previously submitted <b><i>Prha</i> promoter</b> using GFP fluorescence.</li></p>
<li><p><b>Characterized</b> the functionality of our previously submitted <b><i>Prha</i> promoter</b> using GFP fluorescence.</li></p>

Revision as of 19:56, 26 October 2012

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Post-Regional Accomplishments

Our team has had many accomplishments since the regional jamboree!

In our Human Practices project, we...

  • Returned to industry experts to see if we accomplished our goals and what the next steps should be.

  • Characterized the functionality of our previously submitted Prha promoter using GFP fluorescence.

  • Tested an additional complete kill switch device using the Prha promoter with our S7 kill gene.

  • Further characterized our previously validated magnesium riboswitch kill gene construct.


In terms of FRED, we...

  • Electrochemically characterized an inducible lacz circuit, replacing a faulty existing circuit in the registry containing a frameshift.

  • Electrochemically characterized a constitutive bglX generator in terms of its electrochemical activity.

  • Characterized and demonstrated how 3 different hydrolase-producing circuits can be used simultaneously in order to create a multiplex biosensor.

  • Tested one of our transposon library hits with our electrochemical reporter, showing that we can use our system to detect toxins electrochemically.


In terms of OCSAR, we...

  • Obtained additional characterization data in validation of our flux-variability analysis model.

  • Characterized the ability of our novel amidase BioBrick to remove nitrogen atoms from ring structures with remarkeable efficiency, turning them into a substrate that the Petrobrick (BBa_K) can likely convert into hydrocarbons.

  • Tested out our bioreactor and it’s ability to grow cells containing the PetroBrick as well as extract the hydrocarbon layer produced using our belt skimmer