Team:Calgary/Project/OSCAR

From 2012.igem.org

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<p>The <FONT COLOR=43BBFF>O</FONT>ptimized <FONT COLOR=43BBFF>S</FONT>ystem for <FONT COLOR=43BBFF>C</FONT>arboxylic <FONT COLOR=43BBFF>A</FONT>cid <FONT COLOR=43BBFF>R</FONT>emediation, or OSCAR, is the <i><b>Destroy</i></b> component to our iGEM 2012 Calgary project. Building on last years biosensor, OSCAR converts toxic compounds, such as naphthenic acids and catechol, into hydrocarbons by removing unwanted carboxylic acid functional groups from their intricate chemical structures. By creating hydrocarbons we can not only detoxify tailing waters but provide an economically viable method for doing so.  By using flux balance analysis we developed a system to optimize the output of carboxylic acid removal system which we validated in the wetlab.  Furthermore we developed a bioreactor prototype to demonstrate the applicability of our system using novel hydrocarbon collection methodologies.  Finally, we developed constructs and genetic circuits to upgrade these hydrocarbons to reduce sulfur and nitrogen content.  Altogether, OSCAR provides a method to upgrade naphthenic acids and other toxic components from waste products into useable fuels.</p>
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<p>The <FONT COLOR=43BBFF>O</FONT>ptimized <FONT COLOR=43BBFF>S</FONT>ystem for <FONT COLOR=43BBFF>C</FONT>arboxylic <FONT COLOR=43BBFF>A</FONT>cid <FONT COLOR=43BBFF>R</FONT>emediation, or OSCAR, is the <i><b>Destroy</i></b> component to our iGEM 2012 Calgary project. Building on <a href="https://2011.igem.org/Team:Calgary">last year's biosensor</a>, OSCAR converts toxic compounds, such as naphthenic acids and catechol, into hydrocarbons by removing unwanted carboxylic acid functional groups from their intricate chemical structures. By creating hydrocarbons we can not only detoxify tailing waters but provide an economically viable method for doing so.  By using flux balance analysis we developed a system to optimize the output of carboxylic acid removal system which we validated in the wetlab.  Furthermore we developed a bioreactor prototype to demonstrate the applicability of our system using novel hydrocarbon collection methodologies.  Finally, we developed constructs and genetic circuits to upgrade these hydrocarbons to reduce sulfur and nitrogen content.  Altogether, OSCAR provides a method to upgrade naphthenic acids and other toxic components from waste products into useable fuels.</p>
<h2>Overview of OSCAR Removal Pathways ⎯ OSCAR in 4D</h2>
<h2>Overview of OSCAR Removal Pathways ⎯ OSCAR in 4D</h2>

Revision as of 04:40, 1 October 2012

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OSCAR

UCalgary2012 OSCAR+definition.png

The Optimized System for Carboxylic Acid Remediation, or OSCAR, is the Destroy component to our iGEM 2012 Calgary project. Building on last year's biosensor, OSCAR converts toxic compounds, such as naphthenic acids and catechol, into hydrocarbons by removing unwanted carboxylic acid functional groups from their intricate chemical structures. By creating hydrocarbons we can not only detoxify tailing waters but provide an economically viable method for doing so. By using flux balance analysis we developed a system to optimize the output of carboxylic acid removal system which we validated in the wetlab. Furthermore we developed a bioreactor prototype to demonstrate the applicability of our system using novel hydrocarbon collection methodologies. Finally, we developed constructs and genetic circuits to upgrade these hydrocarbons to reduce sulfur and nitrogen content. Altogether, OSCAR provides a method to upgrade naphthenic acids and other toxic components from waste products into useable fuels.

Overview of OSCAR Removal Pathways ⎯ OSCAR in 4D

UCalgary2012 OSCAROverview.png

Decarboxylation

Functional group. Pathway. Progress

Desulfurization

Functional group. Pathway. Progress

Denitrogenation

Functional group. Pathway. Progress

Decatecholization

Functional group. Pathway. Progress

Bioreactor: The House of OSCAR

UCalgary2012 OSCAR Bioreactor.png

Design.Prototype

Flux-Balance Modelling for OSCAR Optimization

Model. Software