Team:Calgary/Project/Accomplish

From 2012.igem.org

(Difference between revisions)
Line 44: Line 44:
<br>
<br>
-
<p><b>In terms of <FONT COLOR=#1088CC>OCSAR</FONT>, we...</b></p>
+
<p><b>In terms of <FONT COLOR=#1088CC>OCSAR, we..</FONT></b></p>
<img src="http://2012.igem.org/wiki/images/c/c3/UCalgary2012_OSCAR_Index_Box.png" style="float: right; padding: 10px;"></img>
<img src="http://2012.igem.org/wiki/images/c/c3/UCalgary2012_OSCAR_Index_Box.png" style="float: right; padding: 10px;"></img>
<ul>
<ul>
-
<li><p>Demonstrated the successful conversion of <b>naphthenic acids into hydrocarbons</b> using Washington 2011's PetroBrick.</p></li>
+
<li><p>Demonstrated the successful conversion of <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/Decarboxylation#Petrobrick">
-
 
+
naphthenic acids into hydrocarbons</a> using Washington 2011's PetroBrick.</p></li>
-
<li><P>Documented the functionality of an <b>alternative enzyme to the PetroBrick</b> (<i>oleT</i>) in producing alkenes from fatty acids.</p></li>
+
-
<li><P>Modified an existing <i>xylE</i> part to show the degradation of <b>catechol into a product, which is then degraded into hydrocarbons</b> using the PetroBrick or OleT enzyme.</p></li>
+
-
<li><P>Designed, built, and tested a <b>functioning bioreactor</b> system in which to house our toxin degrading strain.</p></li>
+
-
<li><P>Used flux variability analysis to <b>optimize the production of our hydrocarbons</b>, surpassing Washington’s previous results through modification of growth media.</p></li>
+
-
<li><P>Demonstrated the successful <b>degradation of carbazole and DBT</b> by our model strains.</p></li>
+
-
<li><P><b>Submitted sequenced BioBricks</b> for the removal of nitrogen and sulfur from various compounds and <b>mutagenized eight separate genes</b> to remove illegal cut sites.</p></li>
+
-
<li><P>Submitted and characterized a <b>new catalase generator</b> as well as a <b>novel oxido-reductase enzyme</b> for use in our desulfurization project.</p></li>
+
-
<li><P><b>Had an amazing summer and learned a ton!</b></p></li></ul>
+
-
 
+
-
</p>
+
 +
<li><P>Documented the functionality of the enzyme <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/Decarboxylation#OleT">OleT</a>, an alternative enzyme to the PetroBrick for producing <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/Decarboxylation#TestingOleT">
 +
alkenes from fatty acids</a>.</p></li>
 +
<li><P>Modified an <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/CatecholDegradation">existing <i>xylE</i></a> part to show the degradation of <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/CatecholDegradation#Catechol">catechol into a product, which is then degraded into hydrocarbons</a> using the PetroBrick or OleT.</p></li>
 +
<li><P>Designed, built, and tested a <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/Bioreactor">functioning bioreactor</a> system in which to house our toxin degrading strain.</p></li>
 +
<li><P>Used flux variability analysis to <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/FluxAnalysis#Flux">
 +
optimize the production of our hydrocarbons</a>, surpassing Washington’s previous results through modification of growth media.</p></li>
 +
<li><P>Demonstrated the successful <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/Desulfurization#Degradation">degradation of carbazole and DBT</a> by our model strains.</p></li>
 +
<li><P>Submitted sequenced BioBricks for the removal of <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/Denitrogenation#nitrogen">nitrogen</a> and <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/Desulfurization#sulfur">
 +
sulfur</a> from various compounds and mutagenized eight separate genes to remove illegal cut sites.</p></li>
 +
<li><P>Submitted and characterized a new <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/Desulfurization#catalase">catalase generator</a> as well as a <a class="blue" href="http://2012.igem.org/Team:Calgary/Project/OSCAR/Desulfurization#catalase">novel oxido-reductase enzyme</a> for use in our desulfurization project.</p></li>
 +
<li><P><b><FONT COLOR=#1088CC>Had an amazing summer and learned a ton!</FONT></b></p></li></ul>
</html>
</html>
}}
}}

Revision as of 20:57, 23 October 2012

Hello! iGEM Calgary's wiki functions best with Javascript enabled, especially for mobile devices. We recommend that you enable Javascript on your device for the best wiki-viewing experience. Thanks!

Accomplishments

Our team had many accomplishments throughout the summer of 2012!

In our Human Practices project, we...


In terms of FRED, we...

  • Constructed a transposon library in Pseudomonas, identifying two positive hits sensitive to a variety of tailings pond toxins.

  • Submitted and electrochemically characterized the function of two novel hydrolase enzymes from E. coli, demonstrating the validity and potential of a triple-output system with high sensitivity and little background noise.

  • Designed and wet-lab verified a kinetic model of electrochemical gene expression.

  • Designed both hardware and software for a biosensor prototype.


In terms of OCSAR, we..