Team:Calgary/Project/Accomplish

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<p> Our Team has had many accomplishments this summer in all aspects of our project.
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In terms of Human Practices, we…
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Established a dialogue between industry experts in order to inform the design of our project.
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Led a discussion through the oil sands leadership initiative (OSLI) on the need and potential for the use of synthetic biology in the oil sands.
 +
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Submitted novel riboswitch, promoter and regulatory elements for use in the tight control of killswitch applications and beyond.
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Submitted and characterized both a magnesium riboswitch/promoter GFP construct and a manganese riboswitch/promoter kill gene construct.
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Showcased our project to our city and the world through various outreach initiatives including a ted talk.
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Premiered and beta tested a video game at the Calgary Telus spark world of science.
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In terms of FRED, we…
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Constructed a transposon library in pseudomonas, identifying two positive hits sensitive to a variety of tailings pond toxins. 
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Submitted and characterized electrochemically the function of two novel hydrolase enzymes from E. coli, demonstrating the validity and potential of a triple-output system with high sensitivity and little background noise.
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Designed and wet-lab verified a kinetic model of electrochemical gene expression.
 +
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Designed both software and hardware for a biosensor prototype.
 +
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In terms of OSCAR, we have…
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Demonstrated the successful conversion of naphthenic acids into hydrocarbons using the PetroBrick.
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Documented the functionality of an alternative enzyme (oleT) in producing alkenes from fatty acids
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Modified an existing xylE part to show the degradation of catechol into, and then its degradation into hydrocarbons using the PetroBrick and oleT enzyme.
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Designed built and tested a functioning bioreactor system in which to house our toxin degrading strain.
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Used flux variability analysis to optimize the production of our hydrocarbons, surpassing Washington’s previous results, through modification of growth media.
 +
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Demonstarted the successful degradation of carbazole and DBT by our model strains.
 +
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Submitted sequenced BioBricks for the removal of nitrogen and sulphur from various compounds.
 +
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Submitted and characterized a new catalase-containing construct as well as a novel oxido-reductase enzyme for use in our desulphurization project.
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Had an amazing summer and learned a ton!
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</p>
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Revision as of 16:04, 3 October 2012

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Data Page

Our Team has had many accomplishments this summer in all aspects of our project. In terms of Human Practices, we… Established a dialogue between industry experts in order to inform the design of our project. Led a discussion through the oil sands leadership initiative (OSLI) on the need and potential for the use of synthetic biology in the oil sands. Submitted novel riboswitch, promoter and regulatory elements for use in the tight control of killswitch applications and beyond. Submitted and characterized both a magnesium riboswitch/promoter GFP construct and a manganese riboswitch/promoter kill gene construct. Showcased our project to our city and the world through various outreach initiatives including a ted talk. Premiered and beta tested a video game at the Calgary Telus spark world of science. In terms of FRED, we… Constructed a transposon library in pseudomonas, identifying two positive hits sensitive to a variety of tailings pond toxins. Submitted and characterized electrochemically the function of two novel hydrolase enzymes from E. coli, demonstrating the validity and potential of a triple-output system with high sensitivity and little background noise. Designed and wet-lab verified a kinetic model of electrochemical gene expression. Designed both software and hardware for a biosensor prototype. In terms of OSCAR, we have… Demonstrated the successful conversion of naphthenic acids into hydrocarbons using the PetroBrick. Documented the functionality of an alternative enzyme (oleT) in producing alkenes from fatty acids Modified an existing xylE part to show the degradation of catechol into, and then its degradation into hydrocarbons using the PetroBrick and oleT enzyme. Designed built and tested a functioning bioreactor system in which to house our toxin degrading strain. Used flux variability analysis to optimize the production of our hydrocarbons, surpassing Washington’s previous results, through modification of growth media. Demonstarted the successful degradation of carbazole and DBT by our model strains. Submitted sequenced BioBricks for the removal of nitrogen and sulphur from various compounds. Submitted and characterized a new catalase-containing construct as well as a novel oxido-reductase enzyme for use in our desulphurization project. Had an amazing summer and learned a ton!