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Team:Calgary/Notebook/Protocols/mgtacircuit
From 2012.igem.org
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TITLE=Characterization of mgtA circuit with S7 promoter| | TITLE=Characterization of mgtA circuit with S7 promoter| | ||
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<li>Cells with the appropriate circuits were grown in LB overnight with 10mM MgCl<font style="text-transform: lowercase;">2</font>.</li> | <li>Cells with the appropriate circuits were grown in LB overnight with 10mM MgCl<font style="text-transform: lowercase;">2</font>.</li> | ||
<li>The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl<font style="text-transform: lowercase;">2</font>.</li> | <li>The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl<font style="text-transform: lowercase;">2</font>.</li> | ||
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<li>Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.</li> | <li>Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.</li> | ||
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</html>}} | </html>}} | ||
Revision as of 03:39, 2 October 2012
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Characterization of mgtA circuit with S7 promoter
- Cells with the appropriate circuits were grown in LB overnight with 10mM MgCl2.
- The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl2.
- Cells were then aliquoted into 96 well plates in triplicates for each concentration and OD was measured at 600nm for every 4 hour for 8 hours.
- Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.
