Team:Calgary/Notebook/Protocols/mgcircuit

From 2012.igem.org

(Difference between revisions)
Line 13: Line 13:
<li> 0.1mM MgCl<sub>2</sub></li>
<li> 0.1mM MgCl<sub>2</sub></li>
<li> 1mM MgCl<sub>2</sub></li>
<li> 1mM MgCl<sub>2</sub></li>
-
<li> 10mM MgCl<sub>2</sub></li><font style="text-transform: lowercase;">2</font>.</li>
+
<li> 10mM MgCl<sub>2</sub></li></ol><font style="text-transform: lowercase;">2</font>.</li>
<li>Cells were then aliquoted into 96 well plates in triplicates for each concentration and florescence was measured every hour for 24 hours at 475nm excitation and 515nm emission.</li>
<li>Cells were then aliquoted into 96 well plates in triplicates for each concentration and florescence was measured every hour for 24 hours at 475nm excitation and 515nm emission.</li>
</ol>
</ol>

Revision as of 05:56, 3 October 2012

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Characterization of the magnesium circuit

  1. Cells containing the appropriate circuits were grown in LB overnight with 10mM MgCl2.
    • mgtAp-rb-K082003
    • mgtAp-rb
    • R0040-mgtArb-K082003
    • R0040-B0034-K082003
  2. The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl
    • 0mM MgCl2
    • 0.01mM MgCl2
    • 0.1mM MgCl2
    • 1mM MgCl2
    • 10mM MgCl2
2.
  • Cells were then aliquoted into 96 well plates in triplicates for each concentration and florescence was measured every hour for 24 hours at 475nm excitation and 515nm emission.