Team:Calgary/Notebook/Protocols/catalase

From 2012.igem.org

(Difference between revisions)
(Created page with "{{Team:Calgary/TemplateNotebookOrange| TITLE=Catalase Assay| CONTENT=<html> <ol> <li>Grow up cultures of J04500-katG+LAA and J04500 overnight at 37*C in LB with appropriate anti...")
Line 8: Line 8:
<li>The following morning, observe culture turbidity.</li>
<li>The following morning, observe culture turbidity.</li>
</ol>
</ol>
 +
 +
<br><br><br><br><br><br><br><br><br><br><br><br><br><br>
</html>}}
</html>}}

Revision as of 02:13, 2 October 2012

Hello! iGEM Calgary's wiki functions best with Javascript enabled, especially for mobile devices. We recommend that you enable Javascript on your device for the best wiki-viewing experience. Thanks!

Catalase Assay

  1. Grow up cultures of J04500-katG+LAA and J04500 overnight at 37*C in LB with appropriate antibiotics.
  2. The following evening, innoculate 20 uL into 3 mL LB with varying concentrations of hydrogen peroxide (0 mM, 1 mM, 5 mM, 10 mM) and grow overnight.
  3. The following morning, observe culture turbidity.